Abstract
Plant meiosis studies have enjoyed a fantastic boom in recent years with the use of Arabidopsis thaliana as an important model species for developmental studies because of its small genome, short life cycle, and large mutant collections. Unlike other eukaryotic models, plant meiosis does not display strict checkpoints and rarely commits to apoptotic processes, which makes it possible to investigate the whole meiotic process (spanning from premeiotic interphase to spore formation) in knockout mutants. In this chapter we describe a protocol for immunolabelling Arabidopsis and Brassica meiotic proteins on robustly spread chromosomes. This protocol allows the detection of a large range of proteins on well-preserved chromosomes and throughout the entire meiotic process.
Keywords
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Fransz P, Armstrong S, Alonso-Blanco C, Fischer TC, Torres-Ruiz RA, Jones GH (1998) Cytogenetics for the model system Arabidopsis thaliana. Plant J 13:867–876
Heslop-Harrison JS (1998) Cytogenetic analysis of Arabidopsis. Methods Mol Biol 82:119–127
Ross KJ, Fransz P, Jones GH (1996) A light microscopic atlas of meiosis in Arabidopsis thaliana. Chromosome Res 4:507–516
Armstrong SJ, Caryl AP, Jones GH, Franklin FCH (2002) Asy1, a protein required for meiotic chromosome synapsis, localizes to axis-associated chromatin in Arabidopsis and Brassica. J Cell Sci 115:3645–3655
Chelysheva L, Grandont L, Vrielynck N, le Guin S, Mercier R, Grelon M (2010) An easy protocol for studying chromatin and recombination protein dynamics during Arabidopsis thaliana meiosis: immunodetection of cohesins, histones and MLH1. Cytogenet Genome Res 129:143–153
Chelysheva L, Diallo S, Vezon D, Gendrot G, Vrielynck N, Belcram K et al (2005) AtREC8 and AtSCC3 are essential to the monopolar orientation of the kinetochores during meiosis. J Cell Sci 118:4621–4632
Cai X, Dong F, Edelmann RE, Makaroff CA (2003) The Arabidopsis SYN1 cohesin protein is required for sister chromatid arm cohesion and homologous chromosome pairing. J Cell Sci 116:2999–3007
Acknowledgments
We thank Wayne Crismani for constructive reading of the manuscript, and C. Franklin and C. Makaroff for providing ASY1 and REC8 antibodies, respectively.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Chelysheva, L.A., Grandont, L., Grelon, M. (2013). Immunolocalization of Meiotic Proteins in Brassicaceae: Method 1. In: Pawlowski, W., Grelon, M., Armstrong, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 990. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-333-6_9
Download citation
DOI: https://doi.org/10.1007/978-1-62703-333-6_9
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-332-9
Online ISBN: 978-1-62703-333-6
eBook Packages: Springer Protocols