Abstract
Chromatin immunoprecipitation (ChIP) is a method that allows identification of chromosomal sites occupied by specific proteins. In this technique, chromatin is extracted from cells, sheared, and, using a specific antibody, enriched in fragments that contain a protein of interest. Genomic location of the protein can then be identified by hybridization of the resulting DNA to tiling microarrays or by sequencing. Thanks to advances in high-throughput sequencing methods, studying protein localization using ChIP has become possible even in species with relatively large genomes. Here, we describe a ChIP protocol that we developed to examine localization of meiotic proteins in maize.
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Acknowledgment
Research to develop this protocol was supported by a grant from National Science Foundation (IOS-1025881) to W.P.P.
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He, Y., Sidhu, G., Pawlowski, W.P. (2013). Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in Maize. In: Pawlowski, W., Grelon, M., Armstrong, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 990. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-333-6_19
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DOI: https://doi.org/10.1007/978-1-62703-333-6_19
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-332-9
Online ISBN: 978-1-62703-333-6
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