Abstract
We have developed a technique that enables the thymidine analogues bromodeoxyuridine (BrdU) or ethynyldeoxyuridine (EdU) to be taken up via the transpiration stream into pollen mother cells and incorporated into the meiotic S-phase. Labelled inflorescences are sampled at intervals, which enables assessment of the progression of labelled meiocytes by detection of the incorporated BrdU or EdU.
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References
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Acknowledgments
The work in our laboratories is supported by the Biotechnology and Biological Sciences Research Council (BBSRC) and the European Commission, FP7 grant 222882. Technical support has been provided by Karen Staples and Steve Price.
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Armstrong, S. (2013). A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana . In: Pawlowski, W., Grelon, M., Armstrong, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 990. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-333-6_12
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DOI: https://doi.org/10.1007/978-1-62703-333-6_12
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-332-9
Online ISBN: 978-1-62703-333-6
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