Abstract
Immunolocalization studies to visualize the distribution of proteins on meiotic chromosomes have become an integral part of studies on meiosis in the model organism Arabidopsis thaliana. This chapter describes fixation, sample preparation, and immunolocalization techniques used in our laboratory. These techniques have been used to visualize a wide range of meiotic proteins involved in different aspects of meiosis, including sister chromatid cohesion, recombination, synapsis, and chromosome segregation.
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References
Chang F, Wang YX, Wang SS, Ma H (2011) Molecular control of microsporogenesis in Arabidopsis. Curr Opin Plant Biol 14:66–73
Cai X, Dong FG, Edelmann RE, Makaroff CA (2003) The Arabidopsis SYN1 cohesin protein is required for sister chromatid arm cohesion and homologous chromosome pairing. J Cell Sci 116:2999–3007
Lam WS, Yang XH, Makaroff CA (2005) Characterization of Arabidopsis thaliana SMC1 and SMC3: evidence that ASMC3 may function beyond chromosome cohesion. J Cell Sci 118: 3037–3048
Armstrong S, Caryl A, Jones G, Franklin F (2002) ASY1, a protein required for meiotic chromosome synpasis, localizes to axis-Âassociated chromatin in Arabidopsis and Brassica. J Cell Sci 115:3645–3655
Armstrong SJ, Franklin FCH, Jones GH (2003) A meiotic time-course for Arabidopsis thaliana. Sex Plant Reprod 16:141–149
Chelysheva L, Grandont L, Vrielynck N, le Guin S, Mercier R, Grelon M (2010) An easy protocol for studying chromatin and recombination protein dynamics during Arabidopsis thaliana meiosis: immunodetection of cohesins, histones and MLH1. Cytogenet Genome Res 129:143–153
Dernburg AF, Sedat JW, Hawley RS (1996) Direct evidence of a role for heterochromatin in meiotic chromosome segregation. Cell 86:135–146
Acknowledgment
Research on meiosis in the Makaroff lab has been supported by a grant (MCB0718191) from the NSF.
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Yang, X., Yuan, L., Makaroff, C.A. (2013). Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana: Method 3. In: Pawlowski, W., Grelon, M., Armstrong, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 990. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-333-6_11
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DOI: https://doi.org/10.1007/978-1-62703-333-6_11
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-332-9
Online ISBN: 978-1-62703-333-6
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