Abstract
Advances in molecular biology and in the genetics of Arabidopsis thaliana have led to it becoming an important model for the analysis of meiosis in plants. Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements for Arabidopsis cytology have provided a range of tools to investigate chromosome behavior during meiosis. This chapter contains a detailed description of cytological techniques currently used in our laboratory for the basic preparation of meiotic chromosomes for investigation of the female and male meiotic pathway and fluorescence in situ hybridization (FISH) analysis for the frequency and distribution of crossovers (chiasmata) at metaphase I.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
Notes
- 1.
Adapted with kind permission from Armstrong, S.J., Sanchez-Moran, E., and Franklin, F.C.H. (2009) Cytological analysis of Arabidopsis thaliana meiotic chromosomes. Methods in Molecular Biology 558: Meiosis Cytological Methods, 131–145.
References
Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796–815
Jones GH, Armstrong SJ, Caryl AP, Franklin FCH (2003) Meiotic chromosome synapsis and recombination in Arabidopsis thaliana; an integration of cytological and molecular approaches. Chromosome Res 11:205–215
Smyth DR, Bowman JL, Meyerowitz EM (1990) Early flower development in Arabidopsis. Plant Cell 2:755–767
Armstrong SJ, Jones GH (2003) Meiotic cytology and chromosome behaviour in wild-type Arabidopsis thaliana. J Exp Bot 54:1–10
Ross KJ, Fransz P, Jones GH (1996) A light microscopic atlas of meiosis in Arabidopsis thaliana. Chromosome Res 4:507–516
Armstrong SJ, Jones GH (2001) Female meiosis in Arabidopsis thaliana and in two meiotic mutants. Sex Plant Reprod 13:177–183
Fransz P, Armstrong SJ, Alonso-Blanco C, Fischer TC, Torrez-Ruiz RA, Jones GH (1998) Cytogenetics for the model system Arabidopsis thaliana. Plant J 13:867–876
Sanchez-Moran E, Armstrong SJ, Santos JL, Franklin FCH, Jones GH (2002) Variation in chiasma frequency among eight accessions of Arabidopsis thaliana. Genetics 162:1415–1422
Campbell BR, Song Y, Posch TE, Cullis CA, Town CD (1992) Sequence and organisation of 5S ribosomal RNA-encoding genes of Arabidopsis thaliana. Gene 112:225–228
Gerlach WL, Bedrock JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7: 1869–1885
Acknowledgements
The work in our laboratory is supported by the Biotechnology and Biological Sciences Research Council (BBSRC) and the European Commission, FP7 grant 222883. Technical support has been provided by Steve Price.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Armstrong, S. (2013). Spreading and Fluorescence In Situ Hybridization of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenetical Analysis. In: Pawlowski, W., Grelon, M., Armstrong, S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 990. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-333-6_1
Download citation
DOI: https://doi.org/10.1007/978-1-62703-333-6_1
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-332-9
Online ISBN: 978-1-62703-333-6
eBook Packages: Springer Protocols