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Melanoblasts as Multipotent Cells in Murine Skin

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Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 989))

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Abstract

Melanoblasts are melanocyte precursors that are derived from neural crest cells (NCCs). Recently we showed that melanoblasts differentiate into not only pigmented melanocytes but also into other NCCs derivatives. Here, we describe methods for the isolation of melanoblasts from mouse skin by flow-cytometry. Methods for culturing the isolated melanoblasts, allowing them to express their multipotentiality, are also described.

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Acknowledgments

This study was supported by a grant from the program Grants-in-Aid for Scientific Research (C) from the Japan Society for Promotion of Science.

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Authors and Affiliations

  1. Department of Tissue and Organ Development, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan

    Tsutomu Motohashi & Takahiro Kunisada

Authors
  1. Tsutomu Motohashi
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  2. Takahiro Kunisada
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Editor information

Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

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Motohashi, T., Kunisada, T. (2013). Melanoblasts as Multipotent Cells in Murine Skin. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 989. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-330-5_15

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  • DOI: https://doi.org/10.1007/978-1-62703-330-5_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-329-9

  • Online ISBN: 978-1-62703-330-5

  • eBook Packages: Springer Protocols

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