Abstract
Brightfield in situ hybridization (BISH) applications have significant advantages over traditional fluorescence in situ hybridization (FISH). BISH slides can be analyzed using a regular microscope while FISH slides require the use of a specialized fluorescence microscope. BISH slides allow observers for correlating the gene status (gene amplifications, gene rearrangements, and gene deletions) and tissue morphology better than FISH slides. Also, BISH slides are ideal for the permanent preservation of gene signals. Furthermore, BISH applications can be optimized using an automated tissue slide processing system. BISH applications are becoming a popular method for clinical examination of gene status for selecting cancer treatments.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Nitta H, Hauss-Wegrzyniak B, Lehrkamp M et al (2008) Development of automated brightfield double in situ hybridization (BISH) application for HER2 gene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence in situ hybridization (FISH). Diagn Pathol 3:41
Stasik CJ, Nitta H, Zhang W et al (2010) Increased MYC gene copy number correlates with increased mRNA levels in diffuse large B-cell lymphoma. Haematologica 95:597–603
Rodig SJ, Kutok JL, Paterson JC et al (2010) The pre-B-cell receptor associated protein VpreB3 is a useful diagnostic marker for identifying c-MYC translocated lymphomas. Haematologica 95:2056–2062
Nitta H, Zhang W, Kelly DB et al (2010) Automated brightfield break-apart in situ hybridization (ba-ISH) application: ALK and MALT1 genes as models. Methods 52:352–358
Kim H, Choe J, Yoo S et al (2011) Detection of ALK gene rearrangement in non-small-cell lung cancer: a comparison of fluorescence in situ hybridization and chromogenic in situ hybridization with correlation of ALK protein expression. J Thorac Oncol 6:1359–1366
Yoshida A, Tsuta K, Nitta H et al (2011) Bright-field dual color chromogenic in situ hybridization for the diagnosis of EML4-ALK-postitive lung adenocarcinomas. J Thorac Oncol 6:1677–1686
Albrecht M (2010) Color blindness. Nat Methods 10:775
Landini G, Perryer DG (2011) More on color blindness. Nat Methods 8:891
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 SpringerScience+Business Media New York
About this protocol
Cite this protocol
Nitta, H., Grogan, T.M. (2013). Bright-Field In Situ Hybridization Methods to Discover Gene Amplifications and Rearrangements in Clinical Samples. In: Moll, J., Colombo, R. (eds) Target Identification and Validation in Drug Discovery. Methods in Molecular Biology, vol 986. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-311-4_21
Download citation
DOI: https://doi.org/10.1007/978-1-62703-311-4_21
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-310-7
Online ISBN: 978-1-62703-311-4
eBook Packages: Springer Protocols