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Extrachromosomal Inducible Expression

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Book cover Dictyostelium discoideum Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 983))

Abstract

Inducible expression systems are very convenient for proteins that induce strong side effects such as retardation of growth or development and are essential for the expression of toxic proteins. In this chapter we describe the doxycycline-inducible expression system, optimized for the controlled expression in. Two types of inducible plasmids are presented, in which transcription is induced by either adding or removing doxycycline, respectively. Detailed protocols are provided for the construction of the plasmids and the inducible expression of the target protein.

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References

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Author information

Authors and Affiliations

  1. Beatson Institute for Cancer Research, Glasgow, UK

    Douwe M. Veltman

  2. Department of Cell Biochemistry, University of Groningen, Groningen, The Netherlands

    Peter J. M. Van Haastert

Authors
  1. Douwe M. Veltman
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  2. Peter J. M. Van Haastert
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Corresponding author

Correspondence to Douwe M. Veltman .

Editor information

Editors and Affiliations

  1. , Centre for Biochemistry, University of Cologne, Joseph-Stelzmann-Str. 52, Köln, 50931, Nordrhein-Westfalen, Germany

    Ludwig Eichinger

  2. , Centre for Biomedical Research, University of Hull, Hull, HU6 7RX, Montserrat

    Francisco Rivero

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© 2013 Springer Science+Business Media, LLC

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Veltman, D.M., Van Haastert, P.J.M. (2013). Extrachromosomal Inducible Expression. In: Eichinger, L., Rivero, F. (eds) Dictyostelium discoideum Protocols. Methods in Molecular Biology, vol 983. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-302-2_14

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  • DOI: https://doi.org/10.1007/978-1-62703-302-2_14

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-301-5

  • Online ISBN: 978-1-62703-302-2

  • eBook Packages: Springer Protocols

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