Capillary Electrophoresis for the Analysis of Glycosaminoglycan-Derived Disaccharides

  • Yuqing Chang
  • Bo Yang
  • Amanda Weyers
  • Robert J. LinhardtEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 984)


Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination, which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapter is a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.

Key words

Glycosaminoglycan Disaccharide 2-Aminoacridone Capillary electrophoresis Laser-induced fluorescence 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Yuqing Chang
    • 1
  • Bo Yang
    • 2
  • Amanda Weyers
    • 1
  • Robert J. Linhardt
    • 1
    Email author
  1. 1.Department of Chemistry and Chemical BiologyCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic InstituteTroyUSA
  2. 2.Key Laboratory of Urbanization and Ecological Restratio, Department of Environmental ScienceEast China Normal UniversityShanghaiChina

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