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Capillary Electrophoresis for the Analysis of Glycosaminoglycan-Derived Disaccharides

  • Yuqing Chang
  • Bo Yang
  • Amanda Weyers
  • Robert J. Linhardt
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 984)

Abstract

Capillary electrophoresis is a common technique used for glycosaminoglycan-derived disaccharide analysis because of its high resolving power, high separation efficiency, high sensitivity, short analysis time, and straightforward operation. CE coupled to laser-induced fluorescence (LIF) detection shows an approximately 100 times higher sensitivity than traditional UV detection at 232 nm. 2-Aminoacridone (AMAC) is a widely used fluorophore for labeling unsaturated disaccharides by deductive amination, which is one of the most important method of derivatization of disaccharides for CE-LIF detection. Outlined in this chapter is a protocol of analyzing glycosaminoglycan-derived disaccharides by CE-LIF with AMAC derivatization.

Key words

Glycosaminoglycan Disaccharide 2-Aminoacridone Capillary electrophoresis Laser-induced fluorescence 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Yuqing Chang
    • 1
  • Bo Yang
    • 2
  • Amanda Weyers
    • 1
  • Robert J. Linhardt
    • 1
  1. 1.Department of Chemistry and Chemical BiologyCenter for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic InstituteTroyUSA
  2. 2.Key Laboratory of Urbanization and Ecological Restratio, Department of Environmental ScienceEast China Normal UniversityShanghaiChina

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