Abstract
Saccharides and chitooligosaccharides can be separated in electrophoretic conditions by raising the pH of the medium, which renders the corresponding alcoholate forms. These anionic species can be separated and detected with capacitively coupled contactless conductivity detection as negative peaks because of their low mobilities when compared to the hydroxyl mobility, which is the main co-ion in the background electrolyte. Three methods for different matrixes are presented in this chapter.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
El Rassi Z (1999) Recent developments in capillary electrophoresis and capillary electrochromatography of carbohydrate species. Electrophoresis 20:3134–3144
Soga T, Serwe M (2000) Determination of carbohydrates in food samples by capillary electrophoresis with indirect UV detection. Food Chem 69:339–344
Hu Q, Zhou TS, Zhang L, Fang YZ (2001) Study of the separation and determination of monosaccharides in soluble coffee by capillary zone electrophoresis with electrochemical detection. Analyst 126:298–301
Campa C, Coslovi A, Flamigni A, Rossi M (2006) Overview on advances in capillary electrophoresis-mass spectrometry of carbohydrates: a tabulated review. Electrophoresis 27:2027–2050
Klampfl CW, Buchberger W (2001) Determination of carbohydrates by capillary electrophoresis with electrospray-mass spectrometric detection. Electrophoresis 22:2737–2742
Carvalho AZ, da Silva JAF, do Lago CL (2003) Determination of mono- and disaccharides by capillary electrophoresis with contactless conductivity detection. Electrophoresis 24:2138–2143
Richter EM, de Jesus DP, Munoz RAA, do Lago CL, Angnes L (2005) Determination of anions, cations, and sugars in coconut water by capillary electrophoresis. J Braz Chem Soc 16:1134–1139
Rainelli A, Hauser PC (2005) Fast electrophoresis in conventional capillaries by employing a rapid injection device and contactless conductivity detection. Anal Bioanal Chem 382:789–794
Blanes L, Saito RM, Genta FA, Donega J, Terra WR, Ferreira C, do Lago CL (2008) Direct detection of underivatized chitooligosaccharides produced through chitinase action using capillary zone electrophoresis. Anal Biochem 373:99–103
Jaros M, Soga T, van de Goor T, Gas B (2005) Conductivity detection in capillary zone electrophoresis: inspection by PeakMaster. Electrophoresis 26:1948–1953
Nogueira T, do Lago CL (2009) Detection of adulterations in processed coffee with cereals and coffee husks using capillary zone electrophoresis. J Sep Sci 32:3507–3511
Tanyanyiwa J, Abad-Villar EM, Hauser PC (2004) Contactless conductivity detection of selected organic ions in on-chip electrophoresis. Electrophoresis 25:903–908
Zemann AJ, Schnell E, Volgger D, Bonn GK (1998) Contactless conductivity detection for capillary electrophoresis. Anal Chem 70:563–567
da Silva JAF, do Lago CL (1998) An oscillometric detector for capillary electrophoresis. Anal Chem 70:4339–4343
Brito-Neto JGA, da Silva JAF, Blanes L, do Lago CL (2005) Understanding capacitively coupled contactless conductivity detection in capillary and microchip electrophoresis. Part 1. Fundam Electroanalysis 17:1198–1206
Brito-Neto JGA, da Silva JAF, Blanes L, do Lago CL (2005) Understanding capacitively coupled contactless conductivity detection in capillary and microchip electrophoresis. Part 2. Peak shape, stray capacitance, noise, and actual electronics. Electroanalysis 17:1207–1214
Francisco KJM, do Lago CL (2009) A compact and high-resolution version of a capacitively coupled contactless conductivity detector. Electrophoresis 30:3458–3464
Epple R, Blanes L, Beavis A, Roux C, Doble P (2010) Analysis of amphetamine-type substances by capillary zone electrophoresis using capacitively coupled contactless conductivity detection. Electrophoresis 31:2608–2613
Kuban P, Hauser PC (2004) Contactless conductivity detection in capillary electrophoresis: a review. Electroanalysis 16:2009–2021
Rendlema JA (1973) Ionization of Carbohydrates in presence of metal-hydroxides and oxides. Adv Chem Series 117:51–69.
Acknowledgment
This work was supported by CNPq grant 471054/2008 and fellowships to TN, RMS, and CLL. LB thanks Australian Future Forensics Network (AFFIN) by financial support.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
do Lago, C.L., Nogueira, T., Blanes, L., Saito, R.M. (2013). Determination of Mono-, Di-, and Oligosaccharides by Capillary Electrophoresis with Capacitively Coupled Contactless Conductivity Detection. In: Volpi, N., Maccari, F. (eds) Capillary Electrophoresis of Biomolecules. Methods in Molecular Biology, vol 984. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-296-4_5
Download citation
DOI: https://doi.org/10.1007/978-1-62703-296-4_5
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-295-7
Online ISBN: 978-1-62703-296-4
eBook Packages: Springer Protocols