Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 984)

Abstract

Reducing monosaccharides were derivatized with 2-aminobenzoic acid (2-AA) through reductive amination using sodium cyanoborohydride as a reductant, and the derivatives were separated by capillary zone electrophoresis with UV detection using 50 mM sodium phosphate (pH 5.5) or 150 mM sodium borate—50 mM sodium phosphate (pH 7.0) running buffer. The derivatives of monosaccharides, which are major components of various carbohydrate materials, were completely separated within 25 min.

Key words

2-Aminobenzoic acid Capillary zone electrophoresis Reductive amination 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Mitsuru Abo
    • 1
  • Li-ping He
    • 2
  • Kae Sato
    • 1
  • Akira Okubo
    • 1
  1. 1.Yokohama College of PharmacyYokohamaJapan
  2. 2.Department of Medical Statistics and Epidemiology, School of Public HealthSun Yat-sen UniversityGuangzhouP.R. China

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