Quantification of Arginine and Dimethylated Arginines in Human Plasma by Field-Amplified Sample Injection Capillary Electrophoresis UV Detection

  • Angelo Zinellu
  • Salvatore Sotgia
  • Luca Deiana
  • Ciriaco Carru
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 984)

Abstract

We report a capillary electrophoresis method by field-amplified sample injection (FASI) for the separation and detection of free plasma arginine and dimethylated arginines. Protein elimination from plasma was performed by acetonitrile/ammonia precipitation. Supernatants were dried, reswollen in water, and directly injected on capillary without complex cleanup by solid-phase extraction and/or tedious sample derivatization procedures. Analytes were baseline-separated within 22 min by using 50 mmol/L Tris phosphate pH 2.3 as running buffer. Due to the stacking effects of electrokinetic injection, it is possible to perform a considerable on-line preconcentration of the analytes before running the electrophoresis. This procedure allows to reach a detection limit in real sample of 10 nmol/L for dimethylated arginines and 20 nmol/L for arginine. Recovery of plasma ADMA was 99–104 % and inter-day CV was less than 3 %.

Key words

Capillary electrophoresis FASI ADMA SDMA arginine 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Angelo Zinellu
    • 1
  • Salvatore Sotgia
    • 2
  • Luca Deiana
    • 2
  • Ciriaco Carru
    • 1
  1. 1.Department Biomedical SciencesUniversity of SassariSassariItaly
  2. 2.Department of Biomedical SciencesUniversity of SassariSassariItaly

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