Abstract
After initial stimulation with antigen and exposure to the growth cytokine interleukin-2, activated T lymphocytes become sensitized to apoptosis upon antigen restimulation through the T cell receptor. This self-regulatory, restimulation-induced cell death (RICD) program constrains the proliferative capacity of activated T cells to help prevent excessive T cell accumulation and associated immunopathology. Here we describe a simple FACS-based approach for measuring RICD sensitivity in activated human T cells following polyclonal restimulation in vitro. This procedure is a straightforward research and clinical diagnostic tool for assessing RICD sensitivity for T cells derived from normal donors and patients suffering from diseases causing dysregulated T cell homeostasis.
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Acknowledgements
This work was supported by grants from Uniformed Services University and the XLP Research Trust.
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© 2013 Springer Science+Business Media New York
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Katz, G., Snow, A.L. (2013). Fluorescence-Activated Cell Sorting-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death in Activated, Primary Human T Cells. In: Snow, A., Lenardo, M. (eds) Immune Homeostasis. Methods in Molecular Biology, vol 979. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-290-2_2
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DOI: https://doi.org/10.1007/978-1-62703-290-2_2
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-289-6
Online ISBN: 978-1-62703-290-2
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