Abstract
In mucins, glycosylation is complex and the most predominant posttranslational modification. Since mucins exhibit differential glycosylation pattern under physiological and pathological conditions, analysis of mucin glycans is important for understanding their specific functions during pathological conditions like cancer. Given the complexity of mucin glycans, several sophisticated analytical tools such as HPLC, mass spectrometry, and lectin sandwich assays are employed for glyco-analysis of mucins. However the specialized expertise and instrumentation required for such analysis are beyond the reach of an average cancer biology laboratory. We described in this chapter the utility of the simple electrophoresis/immunoblotting method to examine the mucin glycan epitopes, using specific antibodies and lectins.
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Acknowledgments
The authors on this work are supported, in part, by grants from the Department of Defense (BC074639, BC083295, and BC09742) and the National Institutes of Health (RO1 CA78590, EDRN UO1 CA111294, RO1 CA133774, RO1 CA131944, SPORE 50 CA127297 and U54 CA163120).
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Gnanapragassam, V.S., Jain, M., Batra, S.K. (2013). Analysis of Tumor-Associated Mucin Glycotopes by Western Transfer Methods. In: Su, G. (eds) Pancreatic Cancer. Methods in Molecular Biology, vol 980. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-287-2_19
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DOI: https://doi.org/10.1007/978-1-62703-287-2_19
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-287-2
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