Abstract
Comprehensive analysis of DNA–protein interactions is important for mapping transcriptional regulatory networks at the genome-wide level. Here, we present a new application of mRNA display, using the in vitro virus (IVV) technology, for in vitro selection of DNA-binding protein complexes. Under optimal selection conditions using bait DNAs, many kinds of DNA-binding protein complexes can be successfully selected from an mRNA display library constructed from poly A+ RNA after several rounds of selection. This mRNA display selection system can identify a variety of DNA-binding protein complexes in a single experiment. Remarkably, this system can also select DNA-binding protein heterooligomeric complexes. Since almost all transcription factors form heterooligomeric complexes to bind with their target DNA, this method should be broadly useful to identify DNA-binding transcription factor complexes.
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Acknowledgments
This work was supported in part by a Grant-in-Aid for Scientific Research, and a Special Coordination Fund from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.
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Tateyama, S., Yanagawa, H. (2013). Application of mRNA Display for In Vitro Selection of DNA-Binding Transcription Factor Complexes. In: Bina, M. (eds) Gene Regulation. Methods in Molecular Biology, vol 977. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-284-1_8
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DOI: https://doi.org/10.1007/978-1-62703-284-1_8
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