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Gene Regulation pp 359–363Cite as

Histone Deacetylase Inhibitor Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 977))

Abstract

Pluripotent stem cells can be directed into myogenic differentiation by small molecular inducers, which preferentially activate muscle-specific transcription networks. Here we describe how to efficiently direct the differentiation of pluripotent P19 cells into skeletal muscle lineage by using histone deacetylase inhibitor, valproic acid and the ligand of retinoic acid receptor, all-trans retinoic acid.

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Acknowledgments

This work was sponsored by operating grant from the Natural Sciences and Engineering Research Council of Canada.

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Authors and Affiliations

  1. Departments of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

    Jihong Chen

  2. Department of Pathology and Laboratory Medicine, University of Ottawa, Ottawa, ON, Canada

    Natascha Lacroix

  3. Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

    Qiao Li

  4. Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada

    Qiao Li

Authors
  1. Jihong Chen
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  2. Natascha Lacroix
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  3. Qiao Li
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Editors and Affiliations

  1. , Department of Chemistry, Purdue University, Oval Drive 560, West Lafayette, 47907-2084, Indiana, USA

    Minou Bina

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Chen, J., Lacroix, N., Li, Q. (2013). Histone Deacetylase Inhibitor Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells. In: Bina, M. (eds) Gene Regulation. Methods in Molecular Biology, vol 977. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-284-1_29

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  • DOI: https://doi.org/10.1007/978-1-62703-284-1_29

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-283-4

  • Online ISBN: 978-1-62703-284-1

  • eBook Packages: Springer Protocols

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