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Comparative Genomic Hybridization of Wilms’ tumor

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Array Comparative Genomic Hybridization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 973))

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Abstract

Cytogenetic analysis of solid tumors including Wilms’ tumor is challenging due to poor chromosome morphology, complexity of abnormalities, and to the possibility of stromal cell overgrowth in tissue culture. Molecular cytogenetic techniques such as chromosomal comparative genomic hybridization (CGH) have improved the diagnosis of chromosomal aberrations in Wilms’ tumor since they can provide results based on the analysis of DNA from nondividing cells. However, chromosomal CGH provides only a limited resolution across the whole genome, which is not different than routine cytogenetic analysis (gains or losses of less than one chromosome band or 10 Mb are not detectable by routine cytogenetics or chromosomal CGH). More recently, the development of genomic arrays opened the possibility of assessing the whole genome at a much higher resolution at a sub-microscopic or sub-band level. Based on the principle of chromosomal CGH, this approach, frequently termed array-CGH, opens the possibility to find invisible changes at the whole genome level not only in abnormal but also in normal tumor karyotypes. Here, we discuss the main technical features, benefits, and limitations of the above three techniques as applied to Wilms’ tumor and summarize the main advances in our knowledge about the genetic changes of Wilms’ tumor and their clinical relevance.

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Acknowledgments

The authors wish to thank the BC Children’s Hospital Telethon grant for funding the Wilms’ tumor chromosomal and array CGH study, Dr Dagmar Kalousek’s research laboratory for developing the chromosomal CGH method, and Dr Suzanne Chan, Chansonette Harvard, and Sally Martell for Wilms’ tumor cytogenetic, chromosomal, and array CGH analysis.

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Authors and Affiliations

  1. Division of Hematology/Oncology/BMT, Department of Pediatrics, British Columbia’s Children’s Hospital, University of British Columbia, Vancouver, BC, Canada

    Shahrad Rod Rassekh

  2. Child and Family Research Institute, Vancouver, BC, Canada

    Shahrad Rod Rassekh

  3. Child and Family Research Institute, and Cytogenetics, BC Children’s Hospital, University of British Columbia, Vancouver, BC, Canada

    Evica Rajcan-Separovic

  4. Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada

    Evica Rajcan-Separovic

Authors
  1. Shahrad Rod Rassekh
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  2. Evica Rajcan-Separovic
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Correspondence to Shahrad Rod Rassekh .

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Editors and Affiliations

  1. , Dept. of Pathology & Lab. Medicine, The Ottawa Hospital, Smyth Road 501, Ottawa, K1H 8L6, Ontario, Canada

    Diponkar Banerjee

  2. , Dept. of Molecular Oncology, BC Cancer Agency, West 10th Avenue 675, Vancouver, V5T 4E6, British Columbia, Canada

    Sohrab P. Shah

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Rassekh, S.R., Rajcan-Separovic, E. (2013). Comparative Genomic Hybridization of Wilms’ tumor. In: Banerjee, D., Shah, S. (eds) Array Comparative Genomic Hybridization. Methods in Molecular Biology, vol 973. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-281-0_16

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  • DOI: https://doi.org/10.1007/978-1-62703-281-0_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-280-3

  • Online ISBN: 978-1-62703-281-0

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