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Array-CGH Analysis of Cutaneous Anaplastic Large Cell Lymphoma

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Array Comparative Genomic Hybridization

Part of the book series: Methods in Molecular Biology ((MIMB,volume 973))

Abstract

This chapter describes a study in which the pattern of numerical chromosomal alterations in cutaneous anaplastic large cell lymphoma (C-ALCL) tumor samples was defined using array-based comparative genomic hybridization (CGH). First, the array-based CGH technique applied is outlined in detail. Next, its application in the analysis of C-ALCL tumor specimens is described. This approach resulted in the identification of highly recurrent chromosomal alterations in C-ALCL that include gain of 7q31 and loss on 6q16-6q21 and 13q34, each affecting 45% of the patients. The pattern characteristic of C-ALCL differs markedly from chromosomal alterations observed in other CTCL such as mycosis fungoides and Sézary syndrome and yielded several candidate genes with potential relevance in the pathogenesis of C-ALCL.

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Acknowledgments

We thank MF Benner, MK Borg, R Dijkman, MH Vermeer, R Willemze, W Zoutman (Department of Dermatology, Leiden University Medical Center (LUMC)); EJ Dreef, Dr PM Jansen (Department of Pathology LUMC); Dr JM Boer (Center for Human and Clinical Genetics, LUMC) and J Knijnenburg (Department of Molecular Cell Biology, LUMC) for their excellent assistance.

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Authors and Affiliations

  1. Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands

    K. Szuhai

  2. Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands

    R. van Doorn, C. P. Tensen &  Van Kester

Authors
  1. K. Szuhai
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  2. R. van Doorn
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  3. C. P. Tensen
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  4. Van Kester
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Corresponding author

Correspondence to C. P. Tensen .

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Editors and Affiliations

  1. , Dept. of Pathology & Lab. Medicine, The Ottawa Hospital, Smyth Road 501, Ottawa, K1H 8L6, Ontario, Canada

    Diponkar Banerjee

  2. , Dept. of Molecular Oncology, BC Cancer Agency, West 10th Avenue 675, Vancouver, V5T 4E6, British Columbia, Canada

    Sohrab P. Shah

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Szuhai, K., van Doorn, R., Tensen, C.P., Van Kester (2013). Array-CGH Analysis of Cutaneous Anaplastic Large Cell Lymphoma. In: Banerjee, D., Shah, S. (eds) Array Comparative Genomic Hybridization. Methods in Molecular Biology, vol 973. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-281-0_13

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  • DOI: https://doi.org/10.1007/978-1-62703-281-0_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-280-3

  • Online ISBN: 978-1-62703-281-0

  • eBook Packages: Springer Protocols

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