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Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma

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Lymphoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 971))

Abstract

Multiparametric flow cytometry is a powerful diagnostic tool that permits rapid assessment of cellular antigen expression to quickly provide immunophenotypic information suitable for disease classification. This chapter describes a general approach for the identification of abnormal lymphoid populations by flow cytometry, including B, T, and Hodgkin lymphoma cells suitable for the clinical and research environment. Knowledge of the common patterns of antigen expression of normal lymphoid cells is critical to permit identification of abnormal populations at disease presentation and for minimal residual disease assessment. We highlight an overview of procedures for processing and immunophenotyping non-Hodgkin B- and T-cell lymphomas and also describe our strategy for the sensitive and specific diagnosis of classical Hodgkin lymphoma.

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Acknowledgement

The authors thank Anju Thomas and the other medical technologists in the Hematopathology laboratory at the University of Washington for their expert technical assistance.

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Authors and Affiliations

  1. Department of Laboratory Medicine, University of Washington, Seattle, WA, USA

    David Wu, Brent L. Wood & Jonathan R. Fromm

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  1. David Wu
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  2. Brent L. Wood
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  3. Jonathan R. Fromm
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Correspondence to Jonathan R. Fromm .

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Editors and Affiliations

  1. Medical School, Institute of Cell Biology (Cancer Resear, University of Duisburg-Essen, Virchowstr. 173, Essen, 45122, Germany

    Ralf Küppers

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Wu, D., Wood, B.L., Fromm, J.R. (2013). Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma. In: Küppers, R. (eds) Lymphoma. Methods in Molecular Biology, vol 971. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-269-8_2

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  • DOI: https://doi.org/10.1007/978-1-62703-269-8_2

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-268-1

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