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Application of Polymeric Surfactants in Chiral Micellar Electrokinetic Chromatography (CMEKC) and CMEKC Coupled to Mass Spectrometry

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Chiral Separations

Part of the book series: Methods in Molecular Biology ((MIMB,volume 970))

Abstract

The use of amino acid-based polymeric surfactants (a.k.a. molecular micelles) in chiral micellar electrokinetic chromatography (CMEKC) has been shown to be a successful separation mode for capillary electrophoresis (CE). In this mode, chiral compounds can be enantioseparated with high efficiency, high chiral selectivity, and versatility. This chapter describes the state-of-the art studies published in the past 5 years in CMEKC using polymeric surfactants. Recent trends in the compatibility of chiral polymeric surfactants with mass spectrometric (MS) detection suggest that this type of chiral selector may be the most promising ones for chiral CE-MS applications. The synthesis of new anionic and cationic MS-compatible polymeric surfactants and their utility in CMEKC and CMEKC-MS are demonstrated. Examples of how to run a typical CMEKC-MS experiment using univariate and multivariate optimization of CMEKC and MS parameters are discussed.

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Acknowledgments

This work was supported by NIH grant (2R01-GM-062314) and, in part, by the American Chemical Society, Petroleum Research Foundation (Grant #PRF-47774-AC7).

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Authors and Affiliations

  1. Department of Chemistry, Center of Biotechnology and Drug Design, Georgia State University, Atlanta, GA, USA

    Jun He & Shahab A. Shamsi

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  1. Jun He
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  2. Shahab A. Shamsi
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Correspondence to Shahab A. Shamsi .

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Editors and Affiliations

  1. , Department of Pharmaceutical Chemistry, Friedrich-Schiller-University Jena, Philosophenweg 14, Jena, 07743, Germany

    Gerhard K. E. Scriba

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He, J., Shamsi, S.A. (2013). Application of Polymeric Surfactants in Chiral Micellar Electrokinetic Chromatography (CMEKC) and CMEKC Coupled to Mass Spectrometry. In: Scriba, G. (eds) Chiral Separations. Methods in Molecular Biology, vol 970. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-263-6_21

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  • DOI: https://doi.org/10.1007/978-1-62703-263-6_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-262-9

  • Online ISBN: 978-1-62703-263-6

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