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mRNA Electroporation as a Tool for Immunomonitoring

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Synthetic Messenger RNA and Cell Metabolism Modulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 969))

Abstract

Monitoring the immune response is an essential aspect of numerous clinical vaccination trials in order to evaluate the efficacy. In these clinical vaccination trials, peripheral blood mononuclear cells (PBMC) are isolated at different time points from patient blood samples and subsequently cryopreserved to allow batch analysis at a later time point. Here, we present a newly developed short-time assay which allows direct ex vivo analysis of multi-epitope antigen-specific immune responses using mRNA electroporation of cryopreserved PBMC. This novel method is a rapid and elegant tool and will be convenient for monitoring the cellular immune status of patients in clinical vaccination settings.

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Acknowledgments

This work was supported by the Interuniversity Attraction Poles (IAP) program #P6/41 financed by the Belgian Science Policy, by research grants of the Foundation Against Cancer (Stichting tegen Kanker), and by a Methusalem grant of the Flemish government and the Antwerp University. NC is a postdoctoral fellow of the Fund for Scientific Research (FWO)-Flanders.

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Authors and Affiliations

  1. Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine & Health Sciences, University of Antwerp, Wilrijk, Belgium

    Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo & Zwi Berneman

Authors
  1. Nathalie Cools
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  2. Kirsten Van Camp
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  3. Viggo Van Tendeloo
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  4. Zwi Berneman
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Corresponding author

Correspondence to Nathalie Cools .

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Editors and Affiliations

  1. The Anlyan Center, Department of Genetics, Yale School of Medicine, Cedar Street 300, New Haven, 06520-8005, Connecticut, USA

    Peter M. Rabinovich

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Cools, N., Van Camp, K., Van Tendeloo, V., Berneman, Z. (2013). mRNA Electroporation as a Tool for Immunomonitoring. In: Rabinovich, P. (eds) Synthetic Messenger RNA and Cell Metabolism Modulation. Methods in Molecular Biology, vol 969. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-260-5_18

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  • DOI: https://doi.org/10.1007/978-1-62703-260-5_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-259-9

  • Online ISBN: 978-1-62703-260-5

  • eBook Packages: Springer Protocols

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