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Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification

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Fungal Diagnostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 968))

Abstract

Rapid and quantitative detection of Aspergillus from clinical samples may facilitate an early diagnosis of invasive pulmonary aspergillosis (IPA). As nucleic acid-based detection is a viable option, we demonstrate that Aspergillus burdens can be rapidly and accurately detected by a novel real-time nucleic acid assay other than qPCR by using the combination of nucleic acid sequence-based amplification (NASBA) and the molecular beacon (MB) technology. Here, we detail a real-time NASBA assay to determine quantitative Aspergillus burdens in lungs and bronchoalveolar lavage (BAL) fluids of rats with experimental IPA.

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Acknowledgments

This work was supported by NIH grant AI066561 to D.S.P.

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Authors and Affiliations

  1. Public Health Research Institute Center, UMDNJ-NJMS, Newark, NJ, USA

    Yanan Zhao & David S. Perlin

Authors
  1. Yanan Zhao
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  2. David S. Perlin
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Corresponding author

Correspondence to David S. Perlin .

Editor information

Editors and Affiliations

  1. , NCBES, Molecular Diagnostics Research Gr, National University of Ireland, Galway, IDA Business Park, Dangan, Galway, Ireland

    Louise O'Connor

  2. , NCBES, Molecular Diagnostics Research Gr, National University of Ireland, Galway, IDA Business Park, Dangan, Galway, Ireland

    Barry Glynn

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Zhao, Y., Perlin, D.S. (2013). Quantitative Detection of Aspergillus spp. by Real-Time Nucleic Acid Sequence-Based Amplification. In: O'Connor, L., Glynn, B. (eds) Fungal Diagnostics. Methods in Molecular Biology, vol 968. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-257-5_6

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  • DOI: https://doi.org/10.1007/978-1-62703-257-5_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-256-8

  • Online ISBN: 978-1-62703-257-5

  • eBook Packages: Springer Protocols

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