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Fungal Diagnostics: Review of Commercially Available Methods

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Fungal Diagnostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 968))

Abstract

Fungi and yeasts are critical causes of acute infection. As such, the detection and identification of these organisms are crucial in the diagnosis of affected patient populations. There is a vast array of commercial tests currently available for diagnostic purposes. These vary from traditional culture and biochemical methods to advanced multiparameter molecular tests. Recent technological advances have driven the development of rapid tests which are complementing and in some cases replacing the more traditional methods of detection. Irrespective of the method used the ultimate goal is timely detection of the infectious agent allowing appropriate treatment and improved outcome for the patient.

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References

  1. Fridkin SK (2005) The changing face of fungal infections in health care settings. Clin Infect Dis 41:1455–60

    Article  PubMed  Google Scholar 

  2. Walsh TJ, Anaissie EJ, Denning DW, Herbrecht R, Kontoyiannis DP, Marr KA, Morrison VA, Segal BH, Steinbach WJ, Stevens DA, van Burik JA, Wingard JR, Patterson TF (2008) Treatment of aspergillosis: clinical practice guidelines of the Infectious Diseases Society of America. Clin Infect Dis 46:327–360

    Article  PubMed  CAS  Google Scholar 

  3. Chandler FW, Kaplan W, Ajello L (1980) Color atlas and text of the histopathology of mycotic diseases. Year Book Medical Publishers, Chicago

    Google Scholar 

  4. Monheit JE, Cowan DF, Moore DG (1984) Rapid detection of fungi in tissue using calcofluor white and fluorescence microscopy. Arch Pathol Lab Med 108:616–618

    PubMed  CAS  Google Scholar 

  5. Nolte FS, Williams JM, Jerris RC, Morello JA, Leitch CD, Matushek S, Schwabe LD, Dorigan F, Kocka FE (1993) Multicenter clinical evaluation of a continuous monitoring blood culture system using fluorescent-sensor technology (BACTEC 9240). J Clin Microbiol 31:552–557

    PubMed  CAS  Google Scholar 

  6. Thorpe TC, Wilson ML, Turner JE, DiGuiseppi JL, Willert M, Mirrett S, Reller LB (1990) BacT/Alert: an automated colorimetric microbial detection system. J Clin Microbiol 28:1608–1612

    PubMed  CAS  Google Scholar 

  7. Mirrett S, Hanson KE, Reller LB (2007) Controlled clinical comparison of VersaTREK and BacT/ALERT blood culture systems. J Clin Microbiol 45:299–302

    Article  PubMed  Google Scholar 

  8. Taschdjian CL, Burchall JJ, Kozinn PJ (1960) Rapid identification of Candida albicans by filamentation on serum and serum substitutes. Am J Dis Child 99:212–215

    CAS  Google Scholar 

  9. Crist AE Jr, Dietz TJ, Kampschroer K (1996) Comparison of the MUREX C. albicans, Albicans-Sure, and BactiCard Candida test kits with the germ tube test for presumptive identification of Candida albicans. J Clin Microbiol 34:2616–2618

    PubMed  CAS  Google Scholar 

  10. Heelan JS, Siliezar D, Coon K (1996) Comparison of rapid testing methods for enzyme production with the germ tube method for presumptive identification of Candida albicans. J Clin Microbiol 34:2847–2849

    PubMed  CAS  Google Scholar 

  11. Carrillo-Muñoz AJ, Quindós G, Cárdenes CD, Alonso-Vargas R, Brió S, Arévalo P, Pemán J, Estivill D, Pontón J (2003) Performance of Bacticard Candida compared with the germ tube test for the presumptive identification of Candida albicans. Mycoses 46:467–470

    Article  PubMed  Google Scholar 

  12. Cárdenes CD, Carrillo-Muñoz AJ, Arias A, Rodríguez-Alvarez C, Torres-Lana A, Sierra A, Arévalo MP (2004) Comparative evaluation of four commercial tests for presumptive identification of Candida albicans. J Microbiol Methods 59:293–297

    Article  PubMed  CAS  Google Scholar 

  13. Fenn JP, Segal H, Blevins L, Fawson S, Newcomb-Gayman P, Carroll KC (1996) Comparison of the Murex Candida albicans CA50 test with germ tube production for identification of C. albicans. Diagn Microbiol Infect Dis 24:31–35

    Article  PubMed  CAS  Google Scholar 

  14. Baalham T, Brown A, Dunsmuir R, Shankland GS, Blackman M (2005) Comparative evaluation of a new biochemical test kit for the presumptive identification of Candida albicans. 15th European Congress of Clinical Microbiology and Infectious Diseases Copenhagen / Denmark. Abstract number: 1134_01_304 http://www.blackwellpublishing.com/eccmid15/abstract.asp?id=36567 Accessed 19 July 2011

  15. Staib F (1962) Cryptococcus neoformans und Guizotia abyssinica (syn. G. oleifera D.C.). Farbreaktion für Cr. neoformans. Z Hyg Infektionskr Med Mikrobiol Immunol Virol 148:466–475

    Google Scholar 

  16. Denning DW, Stevens DA, Hamilton JR (1990) Comparison of Guizotia abyssinica seed extract (birdseed) agar with conventional media for selective identification of Cryptococcus neoformans in patients with acquired immunodeficiency syndrome. J Clin Microbiol 28:2565–2567

    PubMed  CAS  Google Scholar 

  17. Fleming WH, Hopkins JM, Land GA (1977) New culture medium for the presumptive identificaion of Candida albicans and Cryptococcus neoformans. J Clin Microbiol 5: 236–243

    PubMed  CAS  Google Scholar 

  18. Roberts GD, Horstmeier CD, Land GA, Foxworth JH (1978) Rapid urea broth test for yeasts. J Clin Microbiol 7:584–588

    PubMed  CAS  Google Scholar 

  19. Zimmer BL, Roberts GD (1979) Rapid selective urease test for presumptive identification of Cryptococcus neoformans. J Clin Microbiol 10:380–381

    PubMed  CAS  Google Scholar 

  20. Freydière AM, Robert R, Ploton C, Marot-Leblond A, Monerau F, Vandenesch F (2003) Rapid identification of Candida glabrata with a new commercial test, GLABRATA RTT. J Clin Microbiol 41:3861–3963

    Article  PubMed  CAS  Google Scholar 

  21. Freydière AM, Perry JD, Faure O, Willinger B, Tortorano AM, Nicholson A, Peman J, Verweij PE (2004) Routine use of a commercial test, GLABRATA RTT, for rapid identification of Candida glabrata in six laboratories. J Clin Microbiol 42:4870–4872

    Article  PubMed  Google Scholar 

  22. Pemán J, Aparisi N, García-Esteban C, Gobernado M (2004) Rapid identification of Candida glabrata using a new commercial kit. Rev Iberoam Micol 21:82–84

    PubMed  Google Scholar 

  23. Willinger B, Wein S, Hirschl AM, Rotter ML, Manafi M (2005) Comparison of a new commercial test, GLABRATA RTT, with a dipstick test for rapid identification of Candida glabrata. J Clin Microbiol 43:499–501

    Article  PubMed  CAS  Google Scholar 

  24. Fenn JP, Billetdeaux E, Segal H, Skodack-Jones L, Padilla PE, Bale M, Carroll K (1999) Comparison of four methodologies for rapid and cost-effective identification of Candida glabrata. J Clin Microbiol 37:3387–3389

    PubMed  CAS  Google Scholar 

  25. Lopez J, Dalle F, Mantelin P, Moiroux P, Nierlich AC, Pacot A, Cuisenier B, Vagner O, Bonnin A (2001) Rapid identification of Candida glabrata based on trehalose and sucrose assimilation using Rosco diagnostic tablets. J Clin Microbiol 39:1172–1174

    Article  PubMed  CAS  Google Scholar 

  26. Freydière A-M, Parant F, Noel-Baron F, Crepy M, Treny A, Raberin H, Davidson A, Odds FC (2002) Identification of Candida glabrata by a 30-second trehalase test. J Clin Microbiol 40:3602–3605

    Article  PubMed  CAS  Google Scholar 

  27. Murray MP, Zinchuk R, Larone DH (2005) CHROMagar Candida as the sole primary medium for isolation of yeasts and as a source medium for the rapid-assimilation-of-trehalose test. J Clin Microbiol 43: 1210–1212

    Article  PubMed  Google Scholar 

  28. Odds FC, Hanson MF, Davidson AD, Jacobsen MD, Wright P, Whyte JA, Gow NA, Jones BL (2007) One year prospective survey of Candida bloodstream infections in Scotland. J Med Microbiol 56:1066–1075

    Article  PubMed  CAS  Google Scholar 

  29. Orenga S, James AL, Manafi M, Perry JD, Pincus DH (2009) Enzymatic substrates in microbiology. J Microbiol Methods 79: 139–155

    Article  PubMed  CAS  Google Scholar 

  30. Perry JD, Freydière AM (2007) The application of chromogenic media in clinical microbiology. J Appl Microbiol 103:2046–2055

    Article  PubMed  CAS  Google Scholar 

  31. Ghelardi E, Pichierri G, Castagna B, Barnini S, Tavanti A, Campa M (2008) Efficacy of Chromogenic Candida Agar for isolation and presumptive identification of pathogenic yeast species. Clin Microbiol Infect 14: 141–147

    PubMed  CAS  Google Scholar 

  32. Sendid B, François N, Standaert A, Dehecq E, Zerimech F, Camus D, Poulain D (2007) Prospective evaluation of the new chromogenic medium CandiSelect 4 for differentiation and presumptive identification of the major pathogenic Candida species. J Med Microbiol 56:495–499

    Article  PubMed  Google Scholar 

  33. Odds FC, Bernaerts R (1994) CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species. J Clin Microbiol 32:1923–1929

    PubMed  CAS  Google Scholar 

  34. Powell HL, Sand CA, Rennie RP (1998) Evaluation of CHROMagar Candida for presumptive identification of clinically important Candida species. Diagn Microbiol Infect Dis 32:201–204

    Article  PubMed  CAS  Google Scholar 

  35. Baumgartner C, Freydière AM, Gille Y (1996) Direct identification and recognition of yeast species from clinical material by using albicans ID and CHROMagar Candida plates. J Clin Microbiol 34:454–456

    PubMed  CAS  Google Scholar 

  36. Fricker-Hidalgo H, Vandapel O, Duchesne MA, Mazoyer MA, Monget D, Lardy B, Lebeau B, Freney J, Ambroise-Thomas P, Grillot R (1996) Comparison of the new API Candida system to the ID 32C system for identification of clinically important yeast species. J Clin Microbiol 34:1846–1848

    PubMed  CAS  Google Scholar 

  37. Bernal S, Martín Mazuelos E, Chávez M, Coronilla J, Valverde A (1998) Evaluation of the new API Candida system for identification of the most clinically important yeast species. Diagn Microbiol Infect Dis 32:217–221

    Article  PubMed  CAS  Google Scholar 

  38. Buchaille L, Freydière AM, Guinet R, Gille Y (1998) Evaluation of six commercial systems for identification of medically important yeasts. Eur J Clin Microbiol Infect Dis 17:479–488

    PubMed  CAS  Google Scholar 

  39. Campbell CK, Davey KG, Holmes AD, Szekely A, Warnock DW (1999) Comparison of the API Candida system with the AUXACOLOR system for identification of common yeast pathogens. J Clin Microbiol 37:821–823

    PubMed  CAS  Google Scholar 

  40. Paugam A, Benchetrit M, Fiacre A, Tourte-Schaefer C, Dupouy-Camet J (1999) Comparison of four commercialized biochemical systems for clinical yeast identification by colour-producing reactions. Med Mycol 37:11–17

    PubMed  CAS  Google Scholar 

  41. Buesching WJ, Kurek K, Roberts GD (1979) Evaluation of the modified API 20C system for identification of clinically important yeasts. J Clin Microbiol 9:565–569

    PubMed  CAS  Google Scholar 

  42. Land GA, Harrison BA, Hulme KL, Cooper BH, Byrd JC (1979) Evaluation of the new API 20C strip for yeast identification against a conventional method. J Clin Microbiol 10:357–364

    PubMed  CAS  Google Scholar 

  43. Bergan T, Hollum AB, Vangdal M (1982) Evaluation of four commercial biochemical test systems for identification of yeasts. Eur J Clin Microbiol 1:217–222

    Article  PubMed  CAS  Google Scholar 

  44. Schuffenecker I, Freydière A, de Montclos H, Gille Y (1993) Evaluation of four commercial systems for identification of medically important yeasts. Eur J Clin Microbiol Infect Dis 12:255–260

    Article  PubMed  CAS  Google Scholar 

  45. Willemsen M, Breynaert J, Lauwers S (1997) Comparison of Auxacolor with API 20 C Aux in yeast identification. Clin Microbiol Infect 3:369–375

    Article  PubMed  Google Scholar 

  46. Ramani R, Gromadzki S, Pincus DH, Salkin IF, Chaturvedi V (1998) Efficacy of API 20C and ID 32C systems for identification of common and rare clinical yeast isolates. J Clin Microbiol 36:3396–3398

    PubMed  CAS  Google Scholar 

  47. Sand C, Rennie RP (1999) Comparison of three commercial systems for the identification of germ-tube negative yeast species isolated from clinical specimens. Diagn Microbiol Infect Dis 33:223–229

    Article  PubMed  CAS  Google Scholar 

  48. Wadlin JK, Hanko G, Stewart R, Pape J, Nachamkin I (1999) Comparison of three commercial systems for identification of yeasts commonly isolated in the clinical microbiology laboratory. J Clin Mcrobiol 37:1967–1970

    CAS  Google Scholar 

  49. Gündes SG, Gulenc S, Bingol R (2001) Comparative performance of Fungichrom I, Candifast and API 20C Aux systems in the identification of clinically significant yeasts. J Med Microbiol 50:1105–1110

    PubMed  Google Scholar 

  50. Milan EP, Malheiros ES, Fischman O, Colombo AL (1997) Evaluation of the AUXACOLOR system for the identification of clinical yeast isolates. Mycopathologia 137:153–157

    Article  PubMed  CAS  Google Scholar 

  51. Sheppard DC, deSouza E, Hashmi Z, Robson HG, René P (1998) Evaluation of the Auxacolor system for biochemical identification of medically important yeasts. J Clin Microbiol 36:3726–3727

    PubMed  CAS  Google Scholar 

  52. Romney MG, Bryce EA, Rennie RP, Sand CA (2000) Rapid identification of clinical yeast isolates using the colorimetric AUXACOLOR system. Diagn Microbiol Infect Dis 36:137–138

    Article  PubMed  CAS  Google Scholar 

  53. Latouche GN, Daniel HM, Lee OC, Mitchell TG, Sorrell TC, Meyer W (1997) Comparison of use of phenotypic and genotypic characteristics for identification of species of the anamorph genus Candida and related teleomorph yeast species. J Clin Microbiol 35:3171–3180

    PubMed  CAS  Google Scholar 

  54. Marklein G, Josten M, Klanke U, Müller E, Horré R, Maier T, Wenzel T, Kostrzewa M, Bierbaum G, Hoerauf A, Sahl HG (2009) Matrix-assisted laser desorption ionization-time of flight mass spectrometry for fast and reliable identification of clinical yeast isolates. J Clin Microbiol 47:2912–2917

    Article  PubMed  CAS  Google Scholar 

  55. Kitch TT, Jacobs MR, McGinnis MR, Appelbaum PC (1996) Ability of RapID Yeast Plus System to identify 304 clinically significant yeasts within 5 hours. J Clin Microbiol 34:1069–1071

    PubMed  CAS  Google Scholar 

  56. Espinel-Ingroff A, Stockman L, Roberts G, Pincus D, Pollack J, Marler J (1998) Comparison of RapID yeast plus system with API 20C system for identification of common, new, and emerging yeast pathogens. J Clin Microbiol 36:883–886

    PubMed  CAS  Google Scholar 

  57. Heelan JS, Sotomayor E, Coon K, D’Arezzo JB (1998) Comparison of the rapid yeast plus panel with the API20C yeast system for identification of clinically significant isolates of Candida species. J Clin Microbiol 36:1443–1445

    PubMed  CAS  Google Scholar 

  58. Sanguinetti M, Porta R, Sali M, La Sorda M, Pecorini G, Fadda G, Posteraro B (2007) Evaluation of VITEK 2 and RapID Yeast Plus systems for yeast species identification: experience at a large clinical microbiology laboratory. J Clin Microbiol 45:1343–1346

    Article  PubMed  CAS  Google Scholar 

  59. Bowman PI, Ahearn DG (1975) Evaluation of the Uni-Yeast-Tek kit for the identification of medically important yeasts. J Clin Microbiol 2:354–358

    PubMed  CAS  Google Scholar 

  60. Bowman PI, Ahearn DG (1976) Evaluation of commercial systems for the identification of clinical yeast isolates. J Clin Microbiol 4:49–53

    PubMed  CAS  Google Scholar 

  61. Cooper BH, Johnson JB, Thaxton ES (1978) Clinical evaluation of the Uni-Yeast-Tek system for rapid presumptive identification of medically important yeasts. J Clin Microbiol 7:349–355

    PubMed  CAS  Google Scholar 

  62. Salkin IF, Land GA, Hurd NJ, Goldson PR, McGinnis MR (1987) Evaluation of YeastIdent and Uni-Yeast-Tek yeast identification systems. J Clin Microbiol 25: 624–627

    PubMed  CAS  Google Scholar 

  63. McGinnis MR, Molina TC, Pierson DL, Mishra SK (1996) Evaluation of the Biolog MicroStation system for yeast identification. J Med Vet Mycol 34:349–352

    Article  PubMed  CAS  Google Scholar 

  64. Land GA, Salkin IF, El-Zaatari M, McGinnis MR, Hashem G (1991) Evaluation of the Baxter-MicroScan 4-hour enzyme-based yeast identification system. J Clin Microbiol 29: 718–722

    PubMed  CAS  Google Scholar 

  65. St Germain G, Beauchesne D (1991) Evaluation of the MicroScan rapid yeast identification panel. J Clin Microbiol 29:2296–2299

    PubMed  CAS  Google Scholar 

  66. Riddle DL, Giger O, Miller L, Hall GS, Woods GL (1994) Clinical comparison of the Baxter MicroScan yeast identification panel and the Vitek Yeast Biochemical Card. Am J Clin Pathol 101:438–442

    PubMed  CAS  Google Scholar 

  67. Crist AE Jr, Johnson LM, Burke PJ (1996) Evaluation of the Microbial Identification System for identification of clinically isolated yeasts. J Clin Microbiol 34:2408–2410

    PubMed  Google Scholar 

  68. Kellogg JA, Bankert DA, Chaturvedi V (1998) Limitations of the current Microbial Identification System for identification of clinical yeast isolates. J Clin Microbiol 36: 1197–1200

    PubMed  CAS  Google Scholar 

  69. Kellogg JA, Bankert DA, Chaturvedi V (1999) Variation in Microbial Identification System accuracy for yeast identification depending on commercial source of Sabouraud dextrose agar. J Clin Microbiol 37: 2080–2083

    PubMed  CAS  Google Scholar 

  70. Oblack DL, Rhodes JC, Martin WJ (1981) Clinical evaluation of the AutoMicrobic system Yeast Biochemical Card for rapid identification of medically important yeasts. J Clin Microbiol 13:351–355

    PubMed  CAS  Google Scholar 

  71. Land G, Stotler R, Land K, Staneck J (1984) Update and evaluation of the AutoMicrobic yeast identification system. J Clin Microbiol 20:649–652

    PubMed  CAS  Google Scholar 

  72. el-Zaatari M, Pasarell L, McGinnis MR, Buckner J, Land GA, Salkin IF (1990) Evaluation of the updated Vitek yeast identi­fication data base. J Clin Microbiol 28: 1938–1941

    PubMed  CAS  Google Scholar 

  73. Fenn JP, Segal H, Barland B, Denton D, Whisenant J, Chun H, Christofferson K, Hamilton L, Carroll K (1994) Comparison of updated Vitek Yeast Biochemical Card and API 20C yeast identification systems. J Clin Microbiol 32:1184–1187

    PubMed  CAS  Google Scholar 

  74. Dooley DP, Beckius ML, Jeffrey BS (1994) Misidentification of clinical yeast isolates by using the updated Vitek Yeast Biochemical Card. J Clin Microbiol 32:2889–2892

    PubMed  CAS  Google Scholar 

  75. Aubertine CL, Rivera M, Rohan SM, Larone DH (2006) Comparative study of the new colorimetric VITEK 2 yeast identification card fluorometric card and of CHROMagar Candida as a source medium with the new card. J Clin Microbiol 44:227–228

    Article  PubMed  CAS  Google Scholar 

  76. Loïez C, Wallet F, Sendid B, Courcol RJ (2006) Evaluation of VITEK 2 colorimetric cards versus fluorimetric cards for identification of yeasts. Diagn Microbiol Infect Dis 56: 455–457

    Article  PubMed  CAS  Google Scholar 

  77. Hata DJ, Hall L, Fothergill AW, Larone DH, Wengenack NL (2007) Multicenter evaluation of the new VITEK 2 advanced colorimetric yeast identification versus the older card. J Clin Microbiol 45:1087–1092

    Article  PubMed  CAS  Google Scholar 

  78. Valenza G, Strasen J, Schafer F, Frosch M, Kurzai O, Abele-Horn M (2008) Evaluation of new colorimetric Vitek 2 yeast identification card by use of different source media. J Clin Microbiol 46:3784–3787

    Article  PubMed  Google Scholar 

  79. Vijgen S, Nys S, Naesens R, Magerman K, Boel A, Cartuyvels R (2011) Comparison of Vitek identification and antifungal susceptibility testing methods to DNA sequencing and Sensititre YeastOne antifungal testing. Med Mycol 49:107–110

    Article  PubMed  Google Scholar 

  80. Hasyn JJ, Buckley HR (1982) Evaluation of the AutoMicrobic system for identification of yeasts. J Clin Microbiol 16:901–904

    PubMed  CAS  Google Scholar 

  81. Pfaller MA, Preston T, Bale M, Koontz FP, Body BA (1988) Comparison of the Quantum II, API Yeast Ident, and AutoMicrobic systems for identification of clinical yeast isolates. J Clin Microbiol 26:2054–2058

    PubMed  CAS  Google Scholar 

  82. Quiles-Melero I, García-Rodriguez J, Romero-Gómez MP, Gómez-Sánchez P, Mingorance J (2011) Rapid identification of yeasts from positive blood culture bottles by pyrosequencing. Eur J Clin Microbiol Infect Dis 30:21–24

    Article  PubMed  CAS  Google Scholar 

  83. Fukazawa Y (1989) Antigenic structure of Candida albicans. Immunochemical basis of the serologic specificity of the mannans in yeasts. Immunol Ser 47:37–62

    PubMed  CAS  Google Scholar 

  84. Bille J (2010) New nonculture-based methods for the diagnosis of invasive candidiasis. Curr Opin Crit Care 16:460–464

    Article  PubMed  Google Scholar 

  85. Oliveri S, Trovato L, Betta P, Romeo MG, Nicoletti G (2008) Experience with the Platelia Candida ELISA for the diagnosis of invasive candidosis in neonatal patients. Clin Microbiol Infect 14:391–393

    Article  PubMed  CAS  Google Scholar 

  86. Platelia™ Candida AB/AC/AK (2009) Bio-Rad instructions for use. <http://www3.bio-rad.com/cmc_upload/Literature/inserts/62799_881046_GB.pdf> Accessed 01 May 2011

  87. Alam FF, Mustafa AS, Khan ZU (2007) Comparative evaluation of (1, 3)-β-D-glucan, mannan and anti-mannan antibodies, and Candida species-specific snPCR in patients with candidemia. BMC Infect Dis 7:103–111

    Article  PubMed  CAS  Google Scholar 

  88. Arendrup MC, Bergmann OJ, Larsson L, Nielsen HV, Jarløv JO, Christensson B (2010) Detection of candidaemia in patients with and without underlying hematological disease. Clin Microbiol Infect 16:855–862

    PubMed  CAS  Google Scholar 

  89. Sendid B, Caillot D, Baccouch-Humbert B, Klingspor L, Grandjean M, Bonnin A, Poulain D (2003) Contribution of the Platelia Candida-specific antibody and antigen tests to early diagnosis of systemic Candida tropicalis infection in neutropenic adults. J Clin Microbiol 41:4551–4558

    Article  PubMed  Google Scholar 

  90. Mikulska M, Calandra T, Sanguinetti M, Poulain D, Viscoli C, Third European Conference on Infections in Leukemia Group (2010) The use of mannan antigen and anti-mannan antibodies in the diagnosis of invasive candidiasis: recommendations from the Third European Conference on Infections in Leukemia. Crit Care 14:R222

    Google Scholar 

  91. Downey LC, Smith PB, Benjamin DK, Cohen-Wolkowiez M (2010) Recent advances in the detection of neonatal Candidiasis. Curr Fungal Infect Rep 4:17–22

    Article  PubMed  Google Scholar 

  92. Lunel FM, Mennink-Kersten MA, Ruegebrink D, van der Lee HA, Donnelly JP, Blijlevens NM, Verweij PE (2009) Value of Candida serum markers in patients with invasive candidiasis after myeloablative chemotherapy. Diagn Microbiol Infect Dis 64:408–15

    Article  PubMed  CAS  Google Scholar 

  93. Wulf GG, Conradi I, Rüchel R (2004) Diagnostic value of antibody detection against Candida germ tube antigens in a patient with hepatosplenic candidosis. Mycoses 47(Suppl 1):11–14

    Article  PubMed  Google Scholar 

  94. Mitsutake K, Miyazaki T, Tashiro T, Yamamoto Y, Kakeya H, Otsubo T, Kawamura S, Hossain MA, Noda T, Hirakata Y, Kohno S (1996) Enolase antigen, mannan antigen, Cand-Tec antigen, and β-glucan in patients with candidemia. J Clin Microbiol 34:1918–1921

    PubMed  CAS  Google Scholar 

  95. Mori T, Matsumura M (1999) Clinical evaluation of diagnostic methods using plasma and/or serum for three mycoses: aspergillosis, candidosis, and pneumocystosis. Nippon Ishinkin Gakkai Zasshi 40:223–230

    Article  CAS  Google Scholar 

  96. Bär W, Hecker H (2002) Diagnosis of systemic Candida infections in patients of the intensive care unit. Significance of serum antigens and antibodies. Mycoses 45:22–28

    Article  PubMed  Google Scholar 

  97. Pazos C, Moragues MD, Quindós G, Pontón J, Palacio A (2006) Diagnostic potential of (1  →  3)- β-D-glucan and anti-Candida albicans germ tube antibodies for the diagnosis and therapeutic monitoring of invasive candidiasis in neutropenic adult patients. Rev Iberoam Micol 23:209–215

    Article  PubMed  Google Scholar 

  98. Moragues MD, Ortiz N, Iruretagoyena JR, García-Ruiz JC, Amutio E, Rojas A, Mendoza J, Quindós G, Pontón-San Emeterio J (2004) Evaluation of a new commercial test (Candida albicans IFA IgG) for the serodiagnosis of invasive candidiasis. Enferm Infecc Microbiol Clin 22:83–88

    PubMed  Google Scholar 

  99. Pemán J, Zaragoza R, Quindós G, Alkorta M, Cuétara MS, Camarena JJ, Ramírez P, Giménez MJ, Martín-Mazuelos E, Linares-Sicilia MJ, Pontón J, The study group Candida albicans Germ Tube Antibody Detection in Critically Ill Patients (CAGTAUCI) (2011) Clinical factors associated with a Candida albicans germ tube antibody positive test in intensive care unit patients. BMC Infect Dis 11:60–65

    Article  PubMed  Google Scholar 

  100. Zaragoza R, Pemán J, Quindós G, Iruretagoyena JR, Cuétara MS, Ramírez P, Gómez MD, Camarena JJ, Viudes A, Pontón J, On behalf of the study group Candida albicans Germ Tube Antibody Detection in Critically Ill Patients (CAGTAUCI) (2009) Clinical significance of the detection of Candida albicans germ tube-specific antibodies in critically ill patients. Clin Microbiol Infect 15:592–595

    Article  PubMed  CAS  Google Scholar 

  101. Temstet A, Roux P, Poirot JL, Ronin O, Dromer F (1992) Evaluation of a monoclonal antibody-based latex agglutination test for diagnosis of cryptococcosis: comparison with two tests using polyclonal antibodies. J Clin Microbiol 30:2544–2550

    PubMed  CAS  Google Scholar 

  102. Tanner DC, Weinstein MP, Fedorciw B, Joho KL, Thorpe JJ, Reller L (1994) Comparison of commercial kits for detection of cryptococcal antigen. J Clin Microbiol 32:1680–1684

    PubMed  CAS  Google Scholar 

  103. Blevins LB, Fenn J, Segal H, Newcomb-Gayman P, Carroll KC (1995) False-positive cryptococcal antigen latex agglutination caused by disinfectants and soaps. J Clin Microbiol 33:1674–1675

    PubMed  CAS  Google Scholar 

  104. Wilson DA, Sholtis M, Parshall S, Hall GS, Procop GW (2011) False-positive cryptococcal antigen test associated with use of BBL Port-A-Cul transport vials. J Clin Microbiol 49:702–703

    Article  PubMed  Google Scholar 

  105. Heelan JS, Corpus L, Kessimian N (1991) False-positive reactions in the latex agglutination test for Cryptococcus neoformans antigen. J Clin Microbiol 29:1260–1261

    PubMed  CAS  Google Scholar 

  106. McFadden DC, Zaragoza O, Casadevall A (2004) Immunoreactivity of cryptococcal antigen is not stable under prolonged incubations in human serum. J Clin Microbiol 42:2786–2788

    Article  PubMed  CAS  Google Scholar 

  107. Saha DC, Xess I, Biswas A, Bhowmik DM, Padma MV (2009) Detection of Cryptococcus by conventional, serological and molecular methods. J Med Microbiol 58:1098–1105

    Article  PubMed  CAS  Google Scholar 

  108. Jaye DL, Waites KB, Parker B, Bragg SL, Moser SA (1998) Comparison of two rapid latex agglutination tests for detection of cryptococcal capsular polysaccharide. Am J Clin Pathol 109:634–641

    PubMed  CAS  Google Scholar 

  109. Babady NE, Bestrom JE, Jespersen DJ, Jones MF, Beito EM, Binnicker MJ, Wengenack NL (2009) Evaluation of three commercial latex agglutination kits and a commercial enzyme immunoassay for the detection of cryptococcal antigen. Med Mycol 47:336–338

    Article  PubMed  CAS  Google Scholar 

  110. Mennink-Kersten MA, Donnelly JP, Verweij PE (2004) Detection of circulating galactomannan for the diagnosis and management of invasive aspergillosis. Lancet Infect Dis 4:349–57

    Article  PubMed  CAS  Google Scholar 

  111. Verdaguer V, Walsh TJ, Hope W, Cortez KJ (2007) Galactomannan antigen detection in the diagnosis of invasive aspergillosis. Expert Rev Mol Diagn 7:21–32

    Article  PubMed  CAS  Google Scholar 

  112. Thornton CR (2010) Detection of invasive aspergillosis. Adv Appl Microbiol 70:187–216

    Article  PubMed  CAS  Google Scholar 

  113. Chen SC, Kontoyiannis DP (2010) New molecular and surrogate biomarker-based tests in the diagnosis of bacterial and fungal infection in febrile neutropenic patients. Curr Opin Infect Dis 23:567–77

    Article  PubMed  CAS  Google Scholar 

  114. Klont RR, Mennink-Kersten MA, Verweij PE (2004) Utility of Aspergillus antigen detection in specimens other than serum specimens. Clin Infect Dis 39:1467–1474

    Article  PubMed  Google Scholar 

  115. Husain S, Clancy CJ, Nguyen MH, Swartzentruber S, Leather H, LeMonte AM, Durkin MM, Knox KS, Hage CA, Bentsen C, Singh N, Wingard JR, Wheat LJ (2008) Performance characteristics of the Platelia Aspergillus enzyme immunoassay for detection of Aspergillus galactomannan antigen in bronchoalveolar lavage fluid. Clin Vaccine Immunol 15:1760–1763

    Article  PubMed  CAS  Google Scholar 

  116. Hage CA, Knox KS, Davis TE, Wheat LJ (2011) Antigen detection in bronchoalveolar lavage fluid for diagnosis of fungal pneumonia. Curr Opin Pulm Med 17:167–171

    Article  PubMed  CAS  Google Scholar 

  117. Maertens J, Maertens V, Theunissen K, Meersseman W, Meersseman P, Meers S, Verbeken E, Verhoef G, Van Eldere J, Lagrou K (2009) Bronchoalveolar lavage fluid galactomannan for the diagnosis of invasive pulmonary aspergillosis in patients with hematologic diseases. Clin Infect Dis 49: 1688–1693

    Article  PubMed  Google Scholar 

  118. Hsu LY, Ding Y, Phua J, Koh LP, Chan DS, Khoo KL, Tambyah PA (2010) Galactomannan testing of bronchoalveolar lavage fluid is useful for diagnosis of invasive pulmonary aspergillosis in hematology patients. BMC Infect Dis 10:44–49

    Article  PubMed  CAS  Google Scholar 

  119. Nguyen MH, Jaber R, Leather HL, Wingard JR, Staley B, Wheat LJ, Cline CL, Baz M, Rand KH, Clancy CJ (2007) Use of bronchoalveolar lavage to detect galactomannan for diagnosis of pulmonary aspergillosis among nonimmunocompromised hosts. J Clin Microbiol 45:2787–2792

    Article  PubMed  Google Scholar 

  120. Steinbach WJ (2010) Invasive aspergillosis in pediatric patients. Curr Med Res Opin 26: 1779–1787

    Article  PubMed  CAS  Google Scholar 

  121. Verweij PE, Stynen D, Rijs AJ, de Pauw BE, Hoogkamp-Korstanje JA, Meis JF (1995) Sandwich enzyme-linked immunosorbent assay compared with Pastorex latex agglutination test for diagnosing invasive aspergillosis in immunocompromised patients. J Clin Microbiol 33:1912–1914

    PubMed  CAS  Google Scholar 

  122. Machetti M, Feasi M, Mordini N, Van Lint MT, Bacigalupo A, Latgé JP, Sarfati J, Viscoli C (1998) Comparison of an enzyme immunoassay and a latex agglutination system for the diagnosis of invasive aspergillosis in bone marrow transplant recipients. Bone Marrow Transplant 21:917–921

    Article  PubMed  CAS  Google Scholar 

  123. Fricker-Hidalgo H, Coltey B, Llerena C, Renversez JC, Grillot R, Pin I, Pelloux H, Pinel C (2010) Recombinant allergens combined with biological markers in the diagnosis of allergic bronchopulmonary aspergillosis in cystic fibrosis patients. Clin Vaccine Immunol 17:1330–1336

    Article  PubMed  CAS  Google Scholar 

  124. Tsagarakis N, Kentrou N, Margariti V, Malgarinou A, Maurea S, Tsagarakis I, Anastasakou E (2009) Anti-aspergillus antibodies and galactomannan antigen detection for serodiagnosis of aspergillosis. ECCMID Meeting. Abstract number: P905

    Google Scholar 

  125. Stone BA, Clarke AE (1992) Chemistry and biology of (1  →  3)-β-glucans. La Trobe University Press, Melbourne, Australia

    Google Scholar 

  126. Mennink-Kersten MA, Ruegebrink D, Verweij PE (2008) Pseudomonas aeruginosa as a cause of 1,3-β-D-glucan assay reactivity. Clin Infect Dis 46:1930–1931

    Article  PubMed  Google Scholar 

  127. Suzuki T, Ohno N, Saito K, Yadomae T (1992) Activation of the complement system by (1  →  3)-beta-D-glucans having ­different degrees of branching and different ultrastructures. J Pharmacobiodyn 15:277–285

    Article  PubMed  CAS  Google Scholar 

  128. Ohki M, Nakamura T, Morita T, Iwanaga S (1980) A new endotoxin sensitive factor associated with hemolymph coagulation system of horseshoe crab (Limulidae). FEBS Lett 120:217–20

    Article  PubMed  CAS  Google Scholar 

  129. Marty FM, Koo S (2009) Role of (1  →  3)-β-D-glucan in the diagnosis of invasive aspergillosis. Med Mycol 47(Suppl 1):S233–240

    Article  PubMed  CAS  Google Scholar 

  130. Maertens J, Meersseman W, Van Bleyenbergh P (2009) New therapies for fungal pneumonia. Curr Opin Infect Dis 22:183–190

    Article  PubMed  CAS  Google Scholar 

  131. Torelli R, Posteraro B, De Carolis E, De Pascale G, Caira M, Bello G, Antonelli M, Pagano L, Fadda G, Sanguinetti M (2011) Comparison of MycAssay Aspergillus test with galactomannan detection in bronchoalveolar lavage (BAL) fluid samples of hematological and ICU patients. Poster 1941. 21st European society of clinical microbiology and infectious diseases meeting, Milan, Italy

    Google Scholar 

  132. Lehmann LE, Alvarez J, Hunfeld KP, Goglio A, Kost GJ, Louie RF, Raglio A, Regueiro BJ, Wissing H, Stüber F (2009) Potential clinical utility of polymerase chain reaction in microbiological testing for sepsis. Crit Care Med 37:3085–3090

    Article  PubMed  Google Scholar 

  133. von Lilienfeld-Toal M, Lehmann LE, Raadts AD, Hahn-Ast C, Orlopp KS, Marklein G, Purr I, Cook G, Hoeft A, Glasmacher A, Stüber F (2009) Utility of a commercially available multiplex real-time PCR assay to detect bacterial and fungal pathogens in febrile neutropenia. J Clin Microbiol 47:2405–2410

    Article  CAS  Google Scholar 

  134. Mancini N, Clerici D, Diotti R, Perotti M, Ghidoli N, De Marco D, Pizzorno B, Emrich T, Burioni R, Ciceri F, Clementi M (2008) Molecular diagnosis of sepsis in neutropenic patients with hematological malignancies. J Med Microbiol 57:601–604

    Article  PubMed  Google Scholar 

  135. Lamoth F, Jaton K, Prod’hom G, Senn L, Bille J, Calandra T, Marchetti O (2010) Multiplex blood PCR in combination with blood cultures for improvement of microbiological documentation of infection in febrile neutropenia. J Clin Microbiol 48:3510–3516

    Article  PubMed  CAS  Google Scholar 

  136. Dierkes C, Ehrenstein B, Siebig S, Linde HJ, Reischl U, Salzberger B (2009) Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis 9:126–132

    Article  PubMed  CAS  Google Scholar 

  137. Louie RF, Tang Z, Albertson TE, Cohen S, Tran NK, Kost GJ (2008) Multiplex polymerase chain reaction detection enhancement of bacteremia and fungemia. Crit Care Med 36:1487–92

    Article  PubMed  CAS  Google Scholar 

  138. Westh H, Lisby G, Breysse F, Böddinghaus B, Chomarat M, Gant V, Goglio A, Raglio A, Schuster H, Stuber F, Wissing H, Hoeft A (2009) Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect 15:544–51

    Article  PubMed  CAS  Google Scholar 

  139. Maubon D, Hamidfar-Roy R, Courby S, Vesin A, Maurin M, Pavese P, Ravanel N, Bulabois CE, Brion JP, Pelloux H, Timsit JF (2010) Therapeutic impact and diagnostic performance of multiplex PCR in patients with malignancies and suspected sepsis. J Infect 61:335–42

    Article  PubMed  Google Scholar 

  140. Yanagihara K, Kitagawa Y, Tomonaga M, Tsukasaki K, Kohno S, Seki M, Sugimoto H, Shimazu T, Tasaki O, Matsushima A, Ikeda Y, Okamoto S, Aikawa N, Hori S, Obara H, Ishizaka A, Hasegawa N, Takeda J, Kamihira S, Sugahara K, Asari S, Murata M, Kobayashi Y, Ginba H, Sumiyama Y, Kitajima M (2010) Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit. Crit Care 14:R159

    Article  PubMed  Google Scholar 

  141. Sachse S, Straube E, Lehmann M, Bauer M, Rußwurm S, Schmidt K-H (2009) Truncated hCGBP improves NAT-based detection of bacterial species in human samples. J Clin Microbiol 47:1050–1057

    Article  PubMed  CAS  Google Scholar 

  142. Kortgen A, Bloos F, Sachse S, Lehmann M, Straube E, Reinhart K, Bauer M (2009) Clinical relevance of fungal DNAemia detected by multiplex PCR in septic patients. 4th international congress of the german ­sepsis society, Weimar, Germany.

    Google Scholar 

  143. An assessment of the potential clinical utility of a new multiplex-PCR assay (Vyoo®) in the management of ICU patients with sepsis. http://apps.who.int/trialsearch/Trial.aspx?TrialID=DRKS00000612 Accessed 19 July 2011

  144. Wellinghausen N, Kochem AJ, Disqué C, Mühl H, Gebert S, Winter J, Matten J, Sakka SG (2009) Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis. J Clin Microbiol 47:2759–65

    Article  PubMed  CAS  Google Scholar 

  145. Westh H, Lisby G, Breysse F, Böddinghaus B, Chomarat M, Gant V, Goglio A, Raglio A, Schuster H, Stuber F, Wissing H, Hoeft A (2009) Multiplex real-time PCR and blood culture for identification of bloodstream pathogens in patients with suspected sepsis. Clin Microbiol Infect 15:544–51

    Article  PubMed  CAS  Google Scholar 

  146. Maubon D, Hamidfar-Roy R, Courby S, Vesin A, Maurin M, Pavese P, Ravanel N, Bulabois CE, Brion JP, Pelloux H, Timsit JF (2010) Therapeutic impact and diagnostic performance of multiplex PCR in patients with malignancies and suspected sepsis. J Infect 61:335–42

    Article  PubMed  Google Scholar 

  147. Hauser PM, Bille J, Lass-Flörl C, Geltner C, Feldmesser M, Levi M, Patel H, Muggia V, Alexander B, Hughes M, Follett SA, Cui X, Leung F, Morgan G, Moody A, Perlin DS, Denning DW (2011) Multicenter, prospective clinical evaluation of respiratory samples from subjects at risk for Pneumocystis jirovecii infection by use of a commercial real-time PCR assay. J Clin Microbiol 49:1872–8

    Article  PubMed  Google Scholar 

  148. White PL, Perry MD, Loeffler J, Melchers W, Klingspor L, Bretagne S, McCulloch E, Cuenca-Estrella M, Finnstrom N, Donnelly JP, Barnes RA, European Aspergillus PCR Initiative (2010) Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens. J Clin Microbiol. 48:3753–3755

    Google Scholar 

  149. Klingspor L, Loeffler J (2009) Aspergillus PCR formidable challenges and progress. Med Mycol 47(Suppl 1):S241–7

    Article  PubMed  CAS  Google Scholar 

  150. Mengoli C, Cruciani M, Barnes RA, Loeffler J, Donnelly JP (2009) Use of PCR for diagnosis of invasive aspergillosis: systematic review and meta-analysis. Lancet Infect Dis 9:89–96

    Article  PubMed  CAS  Google Scholar 

  151. Baldwin CD, Howe GB, Sampath R, Blyn LB, Matthews H, Harpin V, Hall TA, Drader JJ, Hofstadler SA, Eshoo MW, Rudnick K, Studarus K, Moore D, Abbott S, Janda JM, Whitehouse CA (2009) Usefulness of multilocus polymerase chain reaction followed by electrospray ionization mass spectrometry to identify a diverse panel of bacterial isolates. Diagn Microbiol Infect Dis 63:403–408

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We would like to thank Karine Kaiser, Carole Vachon, and Ira Salkin for their helpful comments and critical review of this manuscript.

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Marcos, J.Y., Pincus, D.H. (2013). Fungal Diagnostics: Review of Commercially Available Methods. In: O'Connor, L., Glynn, B. (eds) Fungal Diagnostics. Methods in Molecular Biology, vol 968. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-257-5_2

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