Abstract
Multiplex, real-time PCR has become an invaluable tool for the rapid identification of pathogens in clinical specimens enabling earlier and more targeted management of antimicrobial therapy. In this chapter, we describe the methodology behind a novel multiplex-tandem PCR (MT-PCR) platform designed for the rapid identification of up to 18 fungal pathogens in blood cultures, primary isolation plates, and whole blood, serum, and plasma.
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References
Lau A et al (2008) Multiplex tandem PCR: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens. J Clin Microbiol 46(9):3021–3027
u A et al (2008) Colony multiplex-tandem PCR for rapid, accurate identification of fungal cultures. J Clin Microbiol 46(12):4058–4060
Lau A et al (2010) Comparison of whole blood, serum and plasma for early detection of candidemia by multiplex-tandem PCR. J Clin Microbiol 48(3):811–816
Stanley KK, Szewczuk E (2005) Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection. Nucleic Acids Res 33(20):e180
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Lau, A., Stanley, K., Sorrell, T. (2013). Multiplex-Tandem PCR for Fungal Diagnostics. In: O'Connor, L., Glynn, B. (eds) Fungal Diagnostics. Methods in Molecular Biology, vol 968. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-257-5_15
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DOI: https://doi.org/10.1007/978-1-62703-257-5_15
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-256-8
Online ISBN: 978-1-62703-257-5
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