Abstract
Multiplex, real-time PCR has become an invaluable tool for the rapid identification of pathogens in clinical specimens enabling earlier and more targeted management of antimicrobial therapy. In this chapter, we describe the methodology behind a novel multiplex-tandem PCR (MT-PCR) platform designed for the rapid identification of up to 18 fungal pathogens in blood cultures, primary isolation plates, and whole blood, serum, and plasma.
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Lau A et al (2008) Multiplex tandem PCR: a novel platform for rapid detection and identification of fungal pathogens from blood culture specimens. J Clin Microbiol 46(9):3021–3027
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Stanley KK, Szewczuk E (2005) Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection. Nucleic Acids Res 33(20):e180
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© 2013 Springer Science+Business Media New York
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Lau, A., Stanley, K., Sorrell, T. (2013). Multiplex-Tandem PCR for Fungal Diagnostics. In: O'Connor, L., Glynn, B. (eds) Fungal Diagnostics. Methods in Molecular Biology, vol 968. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-257-5_15
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DOI: https://doi.org/10.1007/978-1-62703-257-5_15
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-256-8
Online ISBN: 978-1-62703-257-5
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