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JAK-STAT Signalling pp 301–317Cite as

Bacterial Expression, Purification, and Crystallization of Tyrosine Phosphorylated STAT Proteins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 967))

Abstract

Signal Transducer and Activator of Transcription (STAT) proteins are latent cytoplasmic transcription ­factors that become activated by phosphorylation at a C-terminal tyrosine residue. Upon activation STAT proteins translocate to the nucleus and bind to their specific target sites. Here, we describe the recombinant expression of tyrosine phosphorylated STAT proteins in bacteria. This method allows the production of large amounts of activated STAT proteins for structural and biochemical studies including the high-throughput screening of chemical libraries.

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Acknowledgments

We thank former lab members Stefan Becker, Montserrat Soler-Lopez, and Carlo Petosa for setting up the initial protocols for STAT3β and Dd-STATa.

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Authors and Affiliations

  1. European Molecular Biology Laboratory, Heidelberg, Germany

    Florence Baudin & Christoph W. Müller

  2. Unit of Virus Host-Cell Interactions, UJF-EMBL-CNRS UMI 3265, Grenoble, France

    Florence Baudin

Authors
  1. Florence Baudin
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  2. Christoph W. Müller
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Corresponding author

Correspondence to Christoph W. Müller .

Editor information

Editors and Affiliations

  1. Walter and Eliza Hall Institute, Royal Parade 1G, Parkville, 3050, Victoria, Australia

    Sandra E. Nicholson

  2. Walter and Eliza Hall Institute, Royal Parade 1G, Parkville, 3050, Victoria, Australia

    Nicos A. Nicola

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Baudin, F., Müller, C.W. (2013). Bacterial Expression, Purification, and Crystallization of Tyrosine Phosphorylated STAT Proteins. In: Nicholson, S., Nicola, N. (eds) JAK-STAT Signalling. Methods in Molecular Biology, vol 967. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-242-1_21

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  • DOI: https://doi.org/10.1007/978-1-62703-242-1_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-241-4

  • Online ISBN: 978-1-62703-242-1

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