Abstract
Jak binding to cytokine receptors has been shown to be a complex and tight interaction. When studying loss-of-function or gain-of-function mutants of the Jaks or cytokine receptors it is often necessary to know if a certain mutant still associates correctly in the context of the signaling complex. The standard technique to show interaction of Jaks with cytokine receptors or other signalling molecules is Co-immunoprecipitation. Here we describe our protocol and discuss different pitfalls that can be encountered during the procedure.
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Acknowledgements
This work was supported by the University of Luxembourg grants R1F105L01 and R1F107L01.
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Haan, C., Haan, S. (2013). Co-immunoprecipitation Protocol to Investigate Cytokine Receptor-Associated Proteins, e.g., Janus Kinases or Other Associated Signaling Proteins. In: Nicholson, S., Nicola, N. (eds) JAK-STAT Signalling. Methods in Molecular Biology, vol 967. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-242-1_2
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DOI: https://doi.org/10.1007/978-1-62703-242-1_2
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-241-4
Online ISBN: 978-1-62703-242-1
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