Abstract
Phosphorylation of signal transducers and activators of transcription (STATs) indicates their involvement in active signaling. Here we describe immunohistochemical staining procedures for detection and identification of the cellular localization of phospho-STAT2 in the central nervous system (CNS) of mice. The procedures include single and dual/triple labeling immunostaining of phospho-STAT2 in the hippocampus of mice following entorhinal cortex lesion.
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Acknowledgments
This study was supported by grants from the Danish Agency for Science, Technology and Innovation and from Novo Nordisk Fonden.
We thank Dina Dræby and Pia Nyborg Nielsen for their excellent technical support.
The authors thank Dr. Chris Schindler, Columbia University, New York, NY, for providing the STAT2-deficient mice.
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Khorooshi, R., Owens, T. (2013). Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining. In: Nicholson, S., Nicola, N. (eds) JAK-STAT Signalling. Methods in Molecular Biology, vol 967. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-242-1_13
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DOI: https://doi.org/10.1007/978-1-62703-242-1_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-241-4
Online ISBN: 978-1-62703-242-1
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