Abstract
Senescence is associated with changes in gene expression, including the upregulation of stress response- and innate immune response-related genes. In addition, aging animals exhibit characteristic changes in movement behaviors including decreased gait speed and a deterioration in sleep/wake rhythms. Here, we describe methods for tracking Drosophila melanogaster movements in 3D with simultaneous quantification of fluorescent transgenic reporters. This approach allows for the assessment of correlations between behavior, aging, and gene expression as well as for the quantification of biomarkers of aging.
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Acknowledgement
This work was supported by grants from the Department of Health and Human Services to ST (HG02790) and to JT (AG011833). We thank Ravi Prakash for help writing CalibHelp and Anurag Biyani for providing VideoGrabber.
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Ardekani, R., Tavaré, S., Tower, J. (2013). Assessing Senescence in Drosophila Using Video Tracking. In: Galluzzi, L., Vitale, I., Kepp, O., Kroemer, G. (eds) Cell Senescence. Methods in Molecular Biology, vol 965. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-239-1_33
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DOI: https://doi.org/10.1007/978-1-62703-239-1_33
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Publisher Name: Humana Press, Totowa, NJ
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