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Phage Display Selection of Peptides that Target Calcium-Binding Proteins

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Book cover Calcium-Binding Proteins and RAGE

Part of the book series: Methods in Molecular Biology ((MIMB,volume 963))

Abstract

Phage display allows to rapidly identify peptide sequences with binding affinity towards target proteins, for example, calcium-binding proteins (CBPs). Phage technology allows screening of 109 or more independent peptide sequences and can identify CBP binding peptides within 2 weeks. Adjusting of screening conditions allows selecting CBPs binding peptides that are either calcium-dependent or independent. Obtained peptide sequences can be used to identify CBP target proteins based on sequence homology or to quickly obtain peptide-based CBP inhibitors to modulate CBP-target interactions.

The protocol described here uses a commercially available phage display library, in which random 12-mer peptides are displayed on filamentous M13 phages. The library was screened against the calcium-binding protein S100B.

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND, USA

    Stefan W. Vetter

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  1. Stefan W. Vetter
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Correspondence to Stefan W. Vetter .

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Editors and Affiliations

  1. Div of Clinical Chem & Biochem, Department of Pediatrics, University of Zürich, Steinwiesstrasse 75, Zürich, 8032, Switzerland

    Claus W. Heizmann

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Vetter, S.W. (2013). Phage Display Selection of Peptides that Target Calcium-Binding Proteins. In: Heizmann, C. (eds) Calcium-Binding Proteins and RAGE. Methods in Molecular Biology, vol 963. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-230-8_14

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  • DOI: https://doi.org/10.1007/978-1-62703-230-8_14

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-229-2

  • Online ISBN: 978-1-62703-230-8

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