Abstract
Measurement of intracellular Ca2+ concentration ([Ca2+]i) is useful to study the upstream and downstream events of Ca2+ signaling. Ca2+-binding proteins including EF-hand-containing proteins are important downstream effector molecules after an increase of [Ca2+]i. Conversely, these proteins can also act as key modulators for regulation of [Ca2+]i by sensing the Ca2+ levels in the intracellular organelles and cytoplasm. Here we describe a single-cell Ca2+ imaging technique that was used to measure the intracellular Ca2+ levels to examine the function of Ca2+-binding proteins, STIM1 and Calcium release-activated Calcium channel regulator 2A (CRACR2A), using ratiometric Ca2+ dye Fura-2 in adherent and non-adherent cells.
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Acknowledgments
This work was supported by the National Institute of Health grants AI-083432 and AI-088393 to YG.
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Srikanth, S., Gwack, Y. (2013). Measurement of Intracellular Ca2+ Concentration in Single Cells Using Ratiometric Calcium Dyes. In: Heizmann, C. (eds) Calcium-Binding Proteins and RAGE. Methods in Molecular Biology, vol 963. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-230-8_1
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DOI: https://doi.org/10.1007/978-1-62703-230-8_1
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