Abstract
Tissue macrophages and inflammatory neutrophils represent important cells of the innate immune system responsible for various important tasks, i.e., elimination of pathogens and/or granuloma formation. Isolation of large numbers of primary phagocytes is vital for research with these cells. Within this protocol, we present a strategy for isolation of large numbers of inflammatory neutrophils and macrophages from murine skin that allows for follow-up in vivo or in vitro studies.
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Acknowledgments
The present work was supported by GK1043, SFB490, and DFG STE 833/6-1 and 6-2.
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John, D., Fischer, M.R., von Stebut, E. (2013). Model for Generation of Large Numbers of Primary, Inflammatory Skin-Derived Neutrophils, and Macrophages. In: Has, C., Sitaru, C. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 961. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-227-8_27
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DOI: https://doi.org/10.1007/978-1-62703-227-8_27
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-226-1
Online ISBN: 978-1-62703-227-8
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