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Molecular Dermatology pp 363–369Cite as

Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 961))

Abstract

Passive transfer of IgG into neonatal mice is a potential method of reproducing antibody-mediated blistering skin diseases. The major autoantigen for bullous pemphigoid is collagen XVII (COL17)/BP180, which is an epidermal linker transmembrane protein. A single intraperitoneal injection of human or rabbit IgG against pathogenic epitopes for COL17 can induce skin fragility in neonatal mice that express human COL17. Since amino acid sequences of the pathogenic epitopes for COL17 significantly differ between humans and rodents, the required antibodies are those that correctly target the molecule to induce the blistering phenotype.

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Acknowledgments

This work was supported in part by Grant-in-Aid for Young Scientists A (20689021 to W.N.). I wish to thank Ms. Yuko Hayakawa for her technical assistance.

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Authors and Affiliations

  1. Department of Dermatology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan

    Wataru Nishie

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  1. Wataru Nishie
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Correspondence to Wataru Nishie .

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Editors and Affiliations

  1. Universitäts-Hautklinik, Department of Molecular Dermatology, Universitätsklinikum Freiburg, Hauptstr. 7, Freiburg, 79104, Germany

    Cristina Has

  2. Universitäts-Hautklinik, Department of Molecular Dermatology, Universitätklinikum Freiburg, Hauptstr. 7, Freiburg, 79104, Germany

    Cassian Sitaru

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Nishie, W. (2013). Mouse Models of Autoimmune Blistering Diseases Induced by the Passive Transfer of Antibodies. In: Has, C., Sitaru, C. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 961. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-227-8_24

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  • DOI: https://doi.org/10.1007/978-1-62703-227-8_24

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-226-1

  • Online ISBN: 978-1-62703-227-8

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