Abstract
Clarification of the spatiotemporal regulation and function of immune cells within the skin is critical to the understanding of the role of immune cells and the skin in immune homeostasis. Here, we describe a novel assay system for monitoring cell movements in the entire body using the photoconvertible fluorescent protein “Kaede”-transgenic (Tg) mice. We can label immune cells by the change in color of Kaede from green to red in these cells following exposure to violet light and track these cells in the entire body. The Kaede-Tg system is an ideal tool for monitoring precise cellular movements between the skin and other anatomical sites in vivo at different stages of the immune response.
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Acknowledgments
This work was supported in part by the Special Coordination Funds for Promoting Science and Technology of the Japanese Government and in part by Astellas Pharma Inc. in the Formation of Innovation Center for Fusion of Advanced Technologies Program, by Grants–in–Aid for Scientific Research (C) (22590442), and by Grants from Sumitomo Science Foundation.
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Tomura, M., Kabashima, K. (2013). Analysis of Cell Movement Between Skin and Other Anatomical Sites In Vivo Using Photoconvertible Fluorescent Protein “Kaede”-Transgenic Mice. In: Has, C., Sitaru, C. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 961. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-227-8_18
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DOI: https://doi.org/10.1007/978-1-62703-227-8_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-226-1
Online ISBN: 978-1-62703-227-8
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