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Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors

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Book cover Molecular Dermatology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 961))

Abstract

Tumor-infiltrating immune cells have long been thought to affect tumor growth. In recent years, large retrospective studies have shown that the nature and polarization of the immune cells found within the tumor microenvironment impact not only the growth of the primary tumor, but also disease progression and patient survival. This has triggered considerable interest for an in depth analysis of the tumoral immune microenvironment and has created a need for standardized methods to characterize tumor-infiltrating immune cells. Here, we describe three approaches that can be used in mouse and human melanoma tumors.

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Authors and Affiliations

  1. Singapore Immunology Network, BMSI, A-STAR, Singapore, Singapore

    Benjamin Toh, Alessandra Nardin, Xilei Dai, Jo Keeble, Valerie Chew & Jean-Pierre Abastado

Authors
  1. Benjamin Toh
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  2. Alessandra Nardin
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  3. Xilei Dai
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  4. Jo Keeble
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  5. Valerie Chew
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  6. Jean-Pierre Abastado
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Corresponding author

Correspondence to Jean-Pierre Abastado .

Editor information

Editors and Affiliations

  1. Universitäts-Hautklinik, Department of Molecular Dermatology, Universitätsklinikum Freiburg, Hauptstr. 7, Freiburg, 79104, Germany

    Cristina Has

  2. Universitäts-Hautklinik, Department of Molecular Dermatology, Universitätklinikum Freiburg, Hauptstr. 7, Freiburg, 79104, Germany

    Cassian Sitaru

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Toh, B., Nardin, A., Dai, X., Keeble, J., Chew, V., Abastado, JP. (2013). Detection, Enumeration, and Characterization of Immune Cells Infiltrating Melanoma Tumors. In: Has, C., Sitaru, C. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 961. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-227-8_17

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  • DOI: https://doi.org/10.1007/978-1-62703-227-8_17

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-226-1

  • Online ISBN: 978-1-62703-227-8

  • eBook Packages: Springer Protocols

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