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Plant Organogenesis pp 323–332Cite as

Culture Methods and Mutant Generation in the Filamentous Brown Algae Ectocarpus siliculosus

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 959))

Abstract

Ectocarpus siliculosus is a small filamentous alga that has recently emerged as the new model for fundamental research on brown algae. Here, we describe the basic culture protocols for propagating and collecting E. siliculosus material that can then be used in all types of molecular biology, biochemistry and cell biology techniques. In addition, procedures for carrying out genetic experiments (generation of mutants and genetic segregation analyses) on E. siliculosus are described.

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References

  1. Charrier B, Coelho S, Le Bail A et al (2008) Development and physiology of the brown alga Ectocarpus siliculosus: two centuries of research. New Phytol 177(2):319–332

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  4. Le Bail A, Billoud B, Le Panse S et al (2011) ETOILE regulates developmental patterning in the filamentous brown alga Ectocarpus siliculosus. Plant Cell 23(4):1666–1678. doi:10.1105/tpc.110.081919

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Author information

Authors and Affiliations

  1. Cell Biology Division, Department of Biology, University of Erlangen-Nuremberg, Erlangen, Germany

    Aude Le Bail

  2. Morphogenesis of Macroalgae, UMR7139 Marine Plants and Biomolecules, CNRS-UPMC, Station Biologique, Roscoff, France

    Bénédicte Charrier

Authors
  1. Aude Le Bail
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  2. Bénédicte Charrier
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Corresponding author

Correspondence to Bénédicte Charrier .

Editor information

Editors and Affiliations

  1. School of Biosciences, University of Nottingham, Loughborough, LE12 5RD, United Kingdom

    Ive De Smet

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Le Bail, A., Charrier, B. (2013). Culture Methods and Mutant Generation in the Filamentous Brown Algae Ectocarpus siliculosus . In: De Smet, I. (eds) Plant Organogenesis. Methods in Molecular Biology, vol 959. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-221-6_22

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  • DOI: https://doi.org/10.1007/978-1-62703-221-6_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-220-9

  • Online ISBN: 978-1-62703-221-6

  • eBook Packages: Springer Protocols

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