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Measuring Synthesis and Degradation of MHC Class I Molecules

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Antigen Processing

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 960))

Abstract

Major histocompatibility complex (MHC) class I molecules function to present pathogen-derived peptides to cytotoxic T cells or act as ligands for Natural Killer cells, thus alerting the immune system to the presence of invading pathogens. Furthermore MHC class I molecules can be strongly associated with autoimmune diseases. Therefore understanding not only the biosynthesis and the degradation pathways of MHC class I molecules has become important in determining their role in pathogen and autoimmune-related diseases. Here we describe how using epitope-tagged MHC class I molecules can aid in the analysis of MHC class I molecule biosynthesis and degradation and also complement studies using conventional conformationally specific antibodies. Coupled together with pharmacological manipulation which can target both biosynthetic and degradative pathways, this offers a powerful tool in analyzing MHC class I molecules.

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Acknowledgments

D.G. is supported by an Arthritis Research (AR) UK project grant (17222) and a UCL Fast Track Award. A.N.A is supported by an ARUK Fellowship (15293).

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Authors and Affiliations

  1. School of Health, Sport and Bioscience, University of East London, London, UK

    David B. Guiliano

  2. Division of Infection and Immunity/Centre of Rheumatology, Department of Immunology and Molecular Pathology, London, UK

    Antony N. Antoniou

Authors
  1. David B. Guiliano
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  2. Antony N. Antoniou
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Corresponding author

Correspondence to Antony N. Antoniou .

Editor information

Editors and Affiliations

  1. Institut National de la Santé et de la Recherche Médicale, Unité 1013, Université Paris Descartes, Faculté de médecine, Paris, France

    Peter van Endert

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Guiliano, D.B., Antoniou, A.N. (2013). Measuring Synthesis and Degradation of MHC Class I Molecules. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology™, vol 960. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-218-6_8

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  • DOI: https://doi.org/10.1007/978-1-62703-218-6_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-217-9

  • Online ISBN: 978-1-62703-218-6

  • eBook Packages: Springer Protocols

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