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Transgenic Cotton pp 211–222Cite as

Agrobacterium-Mediated Transformation of Cotton (Gossypium hirsutum) Shoot Apex with a Fungal Phytase Gene Improves Phosphorus Acquisition

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 958))

Abstract

Cotton is an important world economic crop plant. It is considered that cotton is recalcitrant to in vitro proliferation. Somatic embryogenesis and plant regeneration has been successful by using hypocotyl, whereas it is highly genotype dependent. Here, a genotype-independent cotton regeneration protocol from shoot apices is presented. Shoot apices from 3- to 5-day-old seedlings of cotton are infected with an Agrobacterium strain, EHA105, carrying the binary vector pC-KSA contained phytase gene (phyA) and the marker gene neomycin phosphotransferase (NPTII), and directly regenerated as shoots in vitro. Rooted shoots can be obtained within 6–8 weeks. Plants that survived by leaf painting kanamycin (kan) were ­further analyzed by DNA and RNA blottings. The transgenic plants with increased the phosphorus (P) acquisition efficiency were obtained following the transformation method.

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Authors and Affiliations

  1. North China Key Laboratory for Crop Germplasm Resources of Education Ministry, Agricultural University of Hebei, Baoding, China

    Zhiying Ma & Xingfen Wang

  2. College of Life Sciences, Hebei University, Baoding, China

    Jianfeng Liu

Authors
  1. Zhiying Ma
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  2. Jianfeng Liu
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  3. Xingfen Wang
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Corresponding author

Correspondence to Zhiying Ma .

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Editors and Affiliations

  1. , Department of Biology, East Carolina University, Howell Science Complex S112, Greenville, 27858, North Carolina, USA

    Baohong Zhang

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Ma, Z., Liu, J., Wang, X. (2013). Agrobacterium-Mediated Transformation of Cotton (Gossypium hirsutum) Shoot Apex with a Fungal Phytase Gene Improves Phosphorus Acquisition. In: Zhang, B. (eds) Transgenic Cotton. Methods in Molecular Biology, vol 958. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-212-4_18

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  • DOI: https://doi.org/10.1007/978-1-62703-212-4_18

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-211-7

  • Online ISBN: 978-1-62703-212-4

  • eBook Packages: Springer Protocols

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