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Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation

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Transgenic Cotton

Part of the book series: Methods in Molecular Biology ((MIMB,volume 958))

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Abstract

microRNAs (miRNAs) are an extensive class of newly identified endogenous small regulatory molecules. Many studies show that miRNAs play a critical role in almost all biological and metabolic progresses through targeting protein-coding genes for mRNA cleavage or translation inhibition. Many miRNAs are also identified from cotton using computational and/or experimental approaches, including the next generation deep sequencing technology. However, the function of the majority of miRNAs are unclear. In this chapter, we describe a detailed method for overexpressing miR 156 in cotton using Agrobacterium-mediated genetic transformation. This provides an approach to investigate the function and regulatory mechanism of miRNAs in cotton.

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Authors and Affiliations

  1. Department of Biology, East Carolina University, Greenville, NC, USA

    Baohong Zhang

  2. Beijing Key Laboratory of Plant Resources Research and Development, Department of Biotechnology, School of Science, Beijing Technology and Business University, Haidian District, Beijing, People’s Republic of China

    Min Wang

  3. Henan Institute of Sciences and Technology, Xinxiang, Henan, China

    Xin Zhang, Chengqi Li & Qinglian Wang

Authors
  1. Baohong Zhang
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  2. Min Wang
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  3. Xin Zhang
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  4. Chengqi Li
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  5. Qinglian Wang
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Corresponding author

Correspondence to Baohong Zhang .

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Editors and Affiliations

  1. , Department of Biology, East Carolina University, Howell Science Complex S112, Greenville, 27858, North Carolina, USA

    Baohong Zhang

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Zhang, B., Wang, M., Zhang, X., Li, C., Wang, Q. (2013). Overexpression of miR 156 in Cotton via Agrobacterium-Mediated Transformation. In: Zhang, B. (eds) Transgenic Cotton. Methods in Molecular Biology, vol 958. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-212-4_16

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  • DOI: https://doi.org/10.1007/978-1-62703-212-4_16

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-211-7

  • Online ISBN: 978-1-62703-212-4

  • eBook Packages: Springer Protocols

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