Abstract
Although well-accepted as the ultimate method for cotton functional genomics, Agrobacterium tumefaciens-mediated cotton transformation is not widely used for functional analyses of cotton genes and their promoters since regeneration of cotton in tissue culture is lengthy and labor intensive. In certain cases, A. rhizogenes-induced hairy root culture has been a suitable molecular tool for functional analyses of genes and promoters for plants that are difficult to regenerate by A. tumefaciens-mediated transformation. Similarly, A. rhizogenes-induced hairy root cultures are an alternative tool for cotton functional genomics. In this chapter, the advantages and disadvantages of using A. rhizogenes-induced cotton hairy root culture over A. tumefaciens-mediated cotton transformation are discussed. The procedures for transformation, generation, selection, and molecular analyses of transgenic cotton hairy roots are introduced by describing the functional analysis of a cotton promoter in cotton hairy roots generated by A. rhizogenes-mediated transformation.
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Acknowledgments
This work was supported by United States Department of Agriculture-Agricultural Research Service project 6435-21000-016-00D. The author acknowledges Stephanie Moss, Cheryl Frankfater, Barbara Triplett, and Michael Dowd of USDA-ARS-Southern Regional Research Center who initiated cotton hairy root cultures to produce gossypol compounds. The author also thanks Dr. Barbara Triplett for critically reading manuscript.
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Kim, H.J. (2013). Agrobacterium rhizogenes-Induced Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics. In: Zhang, B. (eds) Transgenic Cotton. Methods in Molecular Biology, vol 958. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-212-4_15
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DOI: https://doi.org/10.1007/978-1-62703-212-4_15
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