Abstract
Root hairs are highly polarized long tubular outgrowths from the surface of epidermal trichoblast cells. Root hair development is a simple process that has facilitated for the study of cell fate determination and tissue differentiation in higher plants. Root hair patterning types in dicot and monocot plants are different. Rice is a monocot model plant with type II root hair pattern. The method to examine root hair fine structure and cell shape in rice may help elucidate the mechanism of cell initiation and differentiation in monocot plants. Compared with the critical-point-drying SEM, the Cryo-SEM method has great advantage, as the Cryo-SEM can well maintain the delicate structure of root hairs in their natural situation. Here we provide the methodology developed to investigate several rice mutants with impaired root hair cells using Cryo-SEM.
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References
Gilroy S, Jones DL (2000) Through form to function: root hair development and nutrient uptake. Trends Plant Sci 5:56–60
Gahoonia TS, Care D, Nielsen NE (1997) Root hairs and acquisition of phosphorus by wheat and barley cultivars. Plant Soil 191:181–188
Bates TR, Lynch JP (2000) The efficiency of Arabidopsis thaliana (Brassicaceae) root hairs in phosphorus acquisition. Am J Bot 87:964–970
Bates TR, Lynch JP (2000) Plant growth and phosphorus accumulation of wild type and two root hair mutants of Arabidopsis thaliana (Brassicaceae). Am J Bot 87:958–963
Zhou J, Jiao FC, Wu ZC, Li YY, Wang XM, He XW, Zhong WQ, Wu P (2008) OsPHR2 is involved in phosphate-starvation signaling and excessive phosphate accumulation in shoots of plants. Plant Physiol 146:1673–1686
Clowes FAL (2000) Pattern in root meristem development in angiosperms. New Phytol 146:83–94
Dolan L, Costa S (2001) Evolution and genetics of root hair stripes in the root epidermis. J Exp Bot 52:413–417
Kwak SH, Schiefelbein J (2007) The role of the SCRAMBLED receptor-like kinase in patterning the Arabidopsis root epidermis. Dev Biol 302:118–131
Lee MM, Schiefelbein J (1999) WEREWOLF, a MYB-related protein in Arabidopsis, is a position-dependent regulator of epidermal cell patterning. Cell 99:473–483
Schiefelbein JW (2000) Constructing a plant cell. The genetic control of root hair development. Plant Physiol 124:1525–1531
Dolan L, Costa S (2001) Evolution and genetics of root hair stripes in the root epidermis. J Exp Bot 52:413–417
Engvild KC, Rasmussen SK (2004) Root hair mutants of barley. Barley Genet Newsl 34:13–15
Gahoonia TS, Nielsen NE, Joshi PA, Jahoor A (2001) A root hairless barley mutant for elucidating genetic of root hairs and phosphorus uptake. Plant Soil 235:211–219
Suzuki N, Taketa S, Ichii M (2003) Morphological and physiological characteristics of a root-hairless mutant in rice (Oryza sativa L.). Plant Soil 255:9–17
Wen TJ, Schnable PS (1994) Analyses of mutants of three genes that are influence root hair development in Zea mays (Gramineae) suggest that root hairs are dispensable. Am J Bot 81:833–842
Kwasniewski M, Szarejko I (2006) Molecular cloning and characterization of beta-expansin gene related to root hair formation in barley. Plant Physiol 141:1149–1158
Kim CM, Park SH, Je BI, Park SH, Park SJ, Piao HL, Eun MY, Dolan L, Han CD (2007) OsCSLD1, a cellulose synthase-like D1 gene, is required for root hair morphogenesis in rice. Plant Physiol 143:1220–1230
Wen TJ, Hochholdinger F, Sauer M, Bruce W, Schnable PS (2005) The roothairless1 gene of maize encodes a homolog of sec3, which is involved in polar exocytosis. Plant Physiol 138:1637–1643
Hochholdinger F, Wen TJ, Zimmermann R, Chimot-Marolle P, da Costa e Silva O, Bruce W, Lamkey KR, Wienand U, Schnable PS (2008) The maize (Zea mays L.) roothairless3 gene encodes a putative GPI-anchored, monocot-specific, COBRA-like protein that significantly affects grain yield. Plant J 54:888–898
Alto 2100 operators handbook. (2005) GATAN
Instruction manual of Model S-3000N scanning electron microscope (2006) HITACHI
Acknowledgments
This work was supported by the Key Basic Research Special Foundation of China (2005CB120901).
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Yu, Z., Chen, H., Tong, Y., Wu, P. (2013). Analysis of Rice Root Hair Morphology Using Cryo-Scanning Electron Microscopy. In: Yang, Y. (eds) Rice Protocols. Methods in Molecular Biology, vol 956. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-194-3_17
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DOI: https://doi.org/10.1007/978-1-62703-194-3_17
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