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Nuclear Transfer in the Mouse Oocyte

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Mammalian Oocyte Regulation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 957))

Abstract

The nuclear transfer (NT) technique in the mouse has enabled us to generate cloned mice and to establish NT embryonic stem (ntES) cells. Direct nuclear injection into mouse oocytes with a piezo impact drive unit can aid in the bypass of several steps of the original cell fusion procedure. It is important to note that only the NT approach can reveal dynamic and global modifications in the epigenome without using genetic modification as well as generating live animals from single cells. Thus, these techniques could also be applied to the preservation of genetic material from any mouse strain instead of preserving embryos or gametes. Moreover, with this technique, we can use not only living cells but also the nuclei of dead cells from frozen mouse carcasses for NT. This chapter describes our most recent protocols of NT into the mouse oocyte for cloning mice and for the establishment of ntES cells from cloned embryos.

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Authors and Affiliations

  1. Center for Developmental Biology, RIKEN Kobe institute, Kobe, Japan

    Eiji Mizutani & Teruhiko Wakayama

  2. Bioresource Center, RIKEN Tsukuba Institute, Tsukuba, Japan

    Eiji Mizutani & Atsuo Ogura

  3. Faculty of Life and Environmental Sciences, University of Yamanashi, Kofu, Japan

    Eiji Mizutani & Teruhiko Wakayama

Authors
  1. Eiji Mizutani
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  2. Atsuo Ogura
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  3. Teruhiko Wakayama
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Corresponding author

Correspondence to Eiji Mizutani .

Editor information

Editors and Affiliations

  1. Dept. of Cell & Developmental Biology, University College London Institute for Women's Health, London, United Kingdom

    Hayden A. Homer

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Mizutani, E., Ogura, A., Wakayama, T. (2013). Nuclear Transfer in the Mouse Oocyte. In: Homer, H. (eds) Mammalian Oocyte Regulation. Methods in Molecular Biology, vol 957. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-191-2_20

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  • DOI: https://doi.org/10.1007/978-1-62703-191-2_20

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-190-5

  • Online ISBN: 978-1-62703-191-2

  • eBook Packages: Springer Protocols

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