Abstract
We previously isolated Aurora-C (Aurkc/Aie1) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein that plays critical roles in chromosome alignment, segregation, kinetochore-microtubule attachment, and cytokinesis in female mouse meiosis. This chapter describes experimental approaches for examining the subcellular localization and function of Aurora-C kinase during female mouse meiosis, presenting detailed methods for introducing exogenous Aurora-C wild-type and kinase-dead mutant mRNAs into mouse oocytes by cytosolic microinjection, and preparing whole-mount meiotic oocytes and chromosome spreads for confocal immunofluorescence microscopy.
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Yang, KT., Lin, YN., Li, SK., Tang, T.K. (2013). Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes. In: Homer, H. (eds) Mammalian Oocyte Regulation. Methods in Molecular Biology, vol 957. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-191-2_13
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DOI: https://doi.org/10.1007/978-1-62703-191-2_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-190-5
Online ISBN: 978-1-62703-191-2
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