Abstract
Gene silencing techniques have brought new insights into mammalian oocyte and embryo development. More specifically, the use of Morpholino oligonucleotides which sterically inhibit translation from target mRNAs thereby compromising gene function, allowed the identification of important oocyte regulators and especially factors involved in meiotic cell cycle control. Here we describe the method of application of Morpholino oligonucleotides in mouse oocyte research.
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Marangos, P. (2013). Micro-injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes. In: Homer, H. (eds) Mammalian Oocyte Regulation. Methods in Molecular Biology, vol 957. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-191-2_10
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DOI: https://doi.org/10.1007/978-1-62703-191-2_10
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-190-5
Online ISBN: 978-1-62703-191-2
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