Abstract
Principles underlying the recording of high-quality/resolution images of two-dimensional crystals of membrane proteins are discussed in the context of instrumental conditions and operational procedures. A detailed example of low-dose microscope settings is provided along with an overview of a program that implements a computer-aided data acquisition procedure.
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Acknowledgments
Work in the Cheng Laboratory is supported by NIH grants (R01GM082893, R01GM098672, 1S10RR026814-01 and P50 GM082250 (to A. Frankel)) and grants from UCSF Program in Breakthrough Biomedical Research (New Technology Award and Opportunity Award in Basic Science).
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Avila-Sakar, A., Li, X., Zheng, S.Q., Cheng, Y. (2013). Recording High-Resolution Images of Two-Dimensional Crystals of Membrane Proteins. In: Schmidt-Krey, I., Cheng, Y. (eds) Electron Crystallography of Soluble and Membrane Proteins. Methods in Molecular Biology, vol 955. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-176-9_8
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DOI: https://doi.org/10.1007/978-1-62703-176-9_8
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-176-9
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