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Legionella pp 521–540Cite as

The Guinea Pig Model of Legionnaires’ Disease

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 954))

Abstract

The guinea pig pneumonia model mimics Legionnaires’ disease as seen in immunocompromised humans, with high untreated fatality rates and as such lends itself to studies of experimental chemotherapy. Guinea pig infection is also used to assess relative virulence of different Legionella bacterial strains, and has also been used to study host immune defenses. Here I describe the method used to produce Legionella sp. pneumonia in the guinea pig using the intratracheal infection technique. This method uses directly observed intratracheal injection of a bacterial suspension, requiring surgical exposure of the trachea.

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References

  1. Edelstein PH, Calarco K, Yasui VK (1984) Antimicrobial therapy of experimentally induced Legionnaires’ disease in guinea pigs. Am Rev Respir Dis 130:849–856

    PubMed  CAS  Google Scholar 

  2. Edelstein PH, Hu B, Shinzato T, Edelstein MA, Xu W, Bessman MJ (2005) Legionella pneumophila NudA Is a Nudix hydrolase and virulence factor. Infect Immun 73:6567–6576

    Article  PubMed  CAS  Google Scholar 

  3. Berendt RF, Young HW, Allen RG, Knutsen GL (1980) Dose-response of guinea pigs experimentally infected with aerosols of Legionella pneumophila. J Infect Dis 141:186–192

    Article  PubMed  CAS  Google Scholar 

  4. Chandler FW, McDade JE, Hicklin MD, Blackmon JA, Thomason BM, Ewing EP Jr (1979) Pathologic findings in guinea pigs inoculated intraperitoneally with the Legionnaires’ disease bacterium. Ann Intern Med 90:671–675

    PubMed  CAS  Google Scholar 

  5. Fraser DW, Wachsmuth IK, Bopp C, Feeley JC, Tsai TF (1978) Antibiotic treatment of guinea-pigs infected with agent of Legionnaires’ disease. Lancet 1:175–178

    Article  PubMed  CAS  Google Scholar 

  6. Davis GS, Winn WC Jr, Gump DW, Beaty HN (1983) The kinetics of early inflammatory events during experimental pneumonia due to Legionella pneumophila in guinea pigs. J Infect Dis 148:823–835

    Article  PubMed  CAS  Google Scholar 

  7. Gump DW, Davis GS, Winn WC Jr, Beaty HN (1983) Protein and antibody in lavage fluid of guinea pigs with Legionella pneumophila pneumonia. Zentralbl Bakteriol Mikrobiol Hyg [A] 255:145–149

    CAS  Google Scholar 

  8. Davis GS, Winn WC Jr, Gump DW, Craighead JE, Beaty HN (1982) Legionnaires’ pneumonia after aerosol exposure in guinea pigs and rats. Am Rev Respir Dis 126:1050–1057

    PubMed  CAS  Google Scholar 

  9. Winn WC Jr, Davis GS, Gump DW, Craighead JE, Beaty HN (1982) Legionnaires’ pneumonia after intratracheal inoculation of guinea pigs and rats. Lab Invest 47:568–578

    PubMed  Google Scholar 

  10. Davis GS, Winn CW Jr, Gump DW, Craighead JM, Beaty HN (1983) Legionnaires’ pneumonia in guinea pigs and rats produced by aerosol exposure. Chest 83:15S–16S

    Article  PubMed  CAS  Google Scholar 

  11. Baskerville A, Fitzgeorge RB, Broster M, Hambleton P (1983) Histopathology of experimental Legionnaires’ disease in guinea pigs, rhesus monkeys and marmosets. J Pathol 139:349–362

    Article  PubMed  CAS  Google Scholar 

  12. Twisk-Meijssen MJ, Meenhorst PL, van Cronenburg BJ, Mulder JD, Scheffer E, van Furth R (1987) The course of Legionella pneumonia in guinea pigs after inhalation of various quantities of L. pneumophila. Immunobiology 176:108–124

    Article  PubMed  CAS  Google Scholar 

  13. Legionella pneumophila and Legionella-like Agents. (2009) In: Chosewood LC, Wilson DE, (eds.), Biosafety in microbiological and biomedical laboratories (HHS Publication No. CDC 21-1112). 5 edn. U.S. Department of Health and Human Services, Atlanta, Georgia, pp 140–141

    Google Scholar 

  14. Strange RE, Cox CS (1976) Survival of dried and airborne bacteria. In: Gray TRG, Postgate JR, (eds.), The survival of vegetative microbes. England: Cambridge University Press 111–154.

    Google Scholar 

  15. Watanabe M, Nakahama C, Hino J, Soejima R (1985) Legionella pneumophila infection. Studies on the host defense mechanism to aerosol challenge with L. pneumophila. Rinsho Byori 33:618–622

    PubMed  CAS  Google Scholar 

  16. Hambleton P, Broster MG, Dennis PJ, Henstridge R, Fitzgeorge R, Conlan JW (1983) Survival of virulent Legionella pneumophila in aerosols. J Hyg (Lond) 90:451–460

    Article  CAS  Google Scholar 

  17. Dennis PJ, Lee JV (1988) Differences in aerosol survival between pathogenic and non- pathogenic strains of Legionella pneumophila serogroup 1. J Appl Bacteriol 65:135–141

    Article  PubMed  CAS  Google Scholar 

  18. Phalen RF (1984) Methods for exposing animals Inhalation studies: foundations and techniques. CRC Press, Inc, Boca Raton, pp 123–148

    Google Scholar 

  19. Katz SM, Habib WA, Hammel JM, Nash P (1982) Lack of airborne spread of infection by Legionella pneumophila among guinea pigs. Infect Immun 38:620–622

    PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Clinical Microbiology Laboratory, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Paul H. Edelstein

Authors
  1. Paul H. Edelstein
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Corresponding author

Correspondence to Paul H. Edelstein .

Editor information

Editors and Affiliations

  1. , Biology of Intracellular Bacteria, Institut Pasteur, Rue du Dr. Roux 28, Paris, 75724, France

    Carmen Buchrieser

  2. Max von Pettenkofer Institute, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, Munich, 80336, Germany

    Hubert Hilbi

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Edelstein, P.H. (2013). The Guinea Pig Model of Legionnaires’ Disease. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_32

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  • DOI: https://doi.org/10.1007/978-1-62703-161-5_32

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-160-8

  • Online ISBN: 978-1-62703-161-5

  • eBook Packages: Springer Protocols

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