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Characterization of Legionella Lipopolysaccharide

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 954))

Abstract

The lipopolysaccharide(LPS) of Legionella spp. is an immuno-dominant antigen and the basis for Legionella pneumophila serogroup classification. The LPS shows a peculiar structure composed of a very hydrophobic lipid A acylated by long chain fatty acids and an O-antigen-specific chain consisting of homopolymeric legionaminic acid. In this chapter we describe a method for the isolation of LPS from L. pneumophila. In the first part we describe the chemical purification, in the second part we outline the application of monoclonal antibody (mAb) in Western blot and immuno-localization by indirect immunofluorescence. This report does not describe physico-chemical methods that analyze the structure of lipopolysaccharide entities.

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Authors and Affiliations

  1. Institute for Medical Microbiology and Hygiene, University of Technology, Dresden, Germany

    Christian Lück & Jürgen H. Helbig

Authors
  1. Christian Lück
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  2. Jürgen H. Helbig
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Corresponding author

Correspondence to Christian Lück .

Editor information

Editors and Affiliations

  1. , Biology of Intracellular Bacteria, Institut Pasteur, Rue du Dr. Roux 28, Paris, 75724, France

    Carmen Buchrieser

  2. Max von Pettenkofer Institute, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, Munich, 80336, Germany

    Hubert Hilbi

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Lück, C., Helbig, J.H. (2013). Characterization of Legionella Lipopolysaccharide. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_24

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  • DOI: https://doi.org/10.1007/978-1-62703-161-5_24

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-160-8

  • Online ISBN: 978-1-62703-161-5

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