Abstract
The production of outer membrane vesicles (OMVs) is a widespread phenomenon employed by bacteria to secrete cell envelope components into the environment. A contribution of Legionella pneumophila OMVs to the pathogenesis of Legionnaires’ disease is likely due to the high number of virulence-related proteins in the vesicles. OMVs are isolated from the supernatant of liquid cultures of L. pneumophila. After low-speed centrifugation, residual bacteria and cell fragments are eliminated by passing the supernatant through a filter. OMVs are pelleted by ultracentrifugation and resuspended in buffer. The isolated OMVs can be analyzed for their molecular components and their interactions with host structures, bacterial cells, or surfaces.
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This work was supported by DFG grant STE 838/6-1.
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Jäger, J., Steinert, M. (2013). Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila . In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_13
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DOI: https://doi.org/10.1007/978-1-62703-161-5_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-160-8
Online ISBN: 978-1-62703-161-5
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