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Natural Biofilm Formation with Legionella pneumophila

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Legionella

Part of the book series: Methods in Molecular Biology ((MIMB,volume 954))

Abstract

Biofilm formation could be studied in various conditions. Most of the studies with Legionella pneumophila used monospecies biofilm in culture media. In some cases, it is important to study bacteria in conditions more close to environmental conditions. In this paper, we describe protocols to produce natural biofilms from river water that were spiked with L. pneumophila.

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References

  1. Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM (1995) Microbial biofilms. Annu Rev Microbiol 49:711–745

    Article  PubMed  CAS  Google Scholar 

  2. Hindre T, Bruggemann H, Buchrieser C, Héchard Y (2008) Transcriptional profiling of Legionella pneumophila biofilm cells and the influence of iron on biofilm formation. Microbiology 154:30–41

    Article  PubMed  CAS  Google Scholar 

  3. Mampel J, Spirig T, Weber SS, Haagensen JA, Molin S, Hilbi H (2006) Planktonic replication is essential for biofilm formation by Legionella pneumophila in a complex medium under static and dynamic flow conditions. Appl Environ Microbiol 72:2885–2895

    Article  PubMed  CAS  Google Scholar 

  4. Pecastaings S, Berge M, Dubourg KM, Roques C (2010) Sessile Legionella pneumophila is able to grow on surfaces and generate structured monospecies biofilms. Biofouling 26:809–819

    Article  PubMed  CAS  Google Scholar 

  5. Piao Z, Sze CC, Barysheva O, Iida K, Yoshida S (2006) Temperature-regulated formation of mycelial mat-like biofilms by Legionella pneumophila. Appl Environ Microbiol 72:1613–1622

    Article  PubMed  CAS  Google Scholar 

  6. Aurell H, Etienne J, Forey F, Reyrolle M, Girardo P, Farge P, Decludt B, Campese C, Vandenesch F, Jarraud S (2003) Legionella pneumophila serogroup 1 strain Paris: endemic distribution throughout France. J Clin Microbiol 41:3320–3322

    Article  PubMed  Google Scholar 

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Author information

Authors and Affiliations

  1. Faculté de Pharmacie, Laboratoire de Génie Chimique, BioSym Department, Université de Toulouse, Toulouse, France

    Emilie Portier

  2. Laboratoire de Chimie et Microbiologie de l’Eau, Université de Poitiers, Poitiers Cedex, France

    Yann Héchard

Authors
  1. Emilie Portier
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  2. Yann Héchard
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Corresponding author

Correspondence to Yann Héchard .

Editor information

Editors and Affiliations

  1. , Biology of Intracellular Bacteria, Institut Pasteur, Rue du Dr. Roux 28, Paris, 75724, France

    Carmen Buchrieser

  2. Max von Pettenkofer Institute, Ludwig-Maximilians-University, Pettenkoferstrasse 9a, Munich, 80336, Germany

    Hubert Hilbi

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© 2013 Springer Science+Business Media New York

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Portier, E., Héchard, Y. (2013). Natural Biofilm Formation with Legionella pneumophila . In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_11

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  • DOI: https://doi.org/10.1007/978-1-62703-161-5_11

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-160-8

  • Online ISBN: 978-1-62703-161-5

  • eBook Packages: Springer Protocols

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