Abstract
The combination of chemical cross-linking and high-resolution mass spectrometry is an emerging technique for monitoring conformational changes in proteins induced by drug binding. In this chapter, we describe this approach for gaining insights into the conformational changes of the peroxisome proliferator-activated receptor alpha after binding of low-molecular weight ligands. Our strategy provides a basis to efficiently characterize target protein-drug interactions.
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Müller, M.Q., Ihling, C.H., Sinz, A. (2013). Analyzing PPARα/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry. In: Badr, M., Youssef, J. (eds) Peroxisome Proliferator-Activated Receptors (PPARs). Methods in Molecular Biology, vol 952. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-155-4_21
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DOI: https://doi.org/10.1007/978-1-62703-155-4_21
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-62703-155-4
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