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Peroxisome Proliferator-Activated Receptors (PPARs) pp 287–299Cite as

Analyzing PPARα/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 952))

Abstract

The combination of chemical cross-linking and high-resolution mass spectrometry is an emerging technique for monitoring conformational changes in proteins induced by drug binding. In this chapter, we describe this approach for gaining insights into the conformational changes of the peroxisome proliferator-activated receptor alpha after binding of low-molecular weight ligands. Our strategy provides a basis to efficiently characterize target protein-drug interactions.

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther-Universität Halle-Wittenberg, Halle (Saale), Germany

    Mathias Q. Müller, Christian H. Ihling & Andrea Sinz

Authors
  1. Mathias Q. Müller
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  2. Christian H. Ihling
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  3. Andrea Sinz
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Corresponding author

Correspondence to Andrea Sinz .

Editor information

Editors and Affiliations

  1. School of Pharmacy, University of Missouri-Kansas City, Charlotte Street 2464, Kansas City, 64108, USA

    Mostafa Z. Badr

  2. School of Pharmacy, University of Missouri-Kansas City, Charlotte Street 2464, Kansas City, 64108, Missouri, USA

    Jihan A. Youssef

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Müller, M.Q., Ihling, C.H., Sinz, A. (2013). Analyzing PPARα/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry. In: Badr, M., Youssef, J. (eds) Peroxisome Proliferator-Activated Receptors (PPARs). Methods in Molecular Biology, vol 952. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-155-4_21

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  • DOI: https://doi.org/10.1007/978-1-62703-155-4_21

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-154-7

  • Online ISBN: 978-1-62703-155-4

  • eBook Packages: Springer Protocols

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